Isolation and characterization of bacterial polyhydroxybutyrate inclusions
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 2006.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2007
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| Online Access: | http://hdl.handle.net/1721.1/35690 |
| _version_ | 1811091341051953152 |
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| author | Kshetry, Nina |
| author2 | Cho Kyun Rha. |
| author_facet | Cho Kyun Rha. Kshetry, Nina |
| author_sort | Kshetry, Nina |
| collection | MIT |
| description | Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 2006. |
| first_indexed | 2024-09-23T15:00:56Z |
| format | Thesis |
| id | mit-1721.1/35690 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T15:00:56Z |
| publishDate | 2007 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/356902019-04-10T08:01:00Z Isolation and characterization of bacterial polyhydroxybutyrate inclusions Kshetry, Nina Cho Kyun Rha. Massachusetts Institute of Technology. Dept. of Civil and Environmental Engineering. Massachusetts Institute of Technology. Dept. of Civil and Environmental Engineering. Civil and Environmental Engineering. Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 2006. Includes bibliographical references (leaves 79-83). Polyhydroxybutyrate (PHB) is a carbon reserve found in some bacteria, and under nutrient limiting conditions accumulates intracellularly in the form of inclusion bodies. These inclusions contain proteins, and the PHB within the inclusions exists in an amorphous state. In this study a procedure to recover native PHB inclusions was developed, and the isolated inclusions were characterized using 13C NMR, western blotting, SDS-PAGE, atomic force microscopy, and fluorescence microscopy. A model for the structure of native PHB inclusions is proposed. by Nina Kshetry. S.M. 2007-01-10T19:19:09Z 2007-01-10T19:19:09Z 2006 2006 Thesis http://hdl.handle.net/1721.1/35690 71301456 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 83 leaves 22238044 bytes 22237606 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Civil and Environmental Engineering. Kshetry, Nina Isolation and characterization of bacterial polyhydroxybutyrate inclusions |
| title | Isolation and characterization of bacterial polyhydroxybutyrate inclusions |
| title_full | Isolation and characterization of bacterial polyhydroxybutyrate inclusions |
| title_fullStr | Isolation and characterization of bacterial polyhydroxybutyrate inclusions |
| title_full_unstemmed | Isolation and characterization of bacterial polyhydroxybutyrate inclusions |
| title_short | Isolation and characterization of bacterial polyhydroxybutyrate inclusions |
| title_sort | isolation and characterization of bacterial polyhydroxybutyrate inclusions |
| topic | Civil and Environmental Engineering. |
| url | http://hdl.handle.net/1721.1/35690 |
| work_keys_str_mv | AT kshetrynina isolationandcharacterizationofbacterialpolyhydroxybutyrateinclusions |