Aggregation of Synthetic Gene Delivery Vectors Enhance the Cellular Association and Uptake for in vitro Transfection
Development of safe and efficient synthetic gene delivery vectors is hampered with limited understanding the fundamental correlation of physicochemical properties of the vectors with their biological activities. Five major barriers contributing to poor transfection efficiency of synthetic vectors in...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2007
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| Subjects: | |
| Online Access: | http://hdl.handle.net/1721.1/35871 |
| _version_ | 1811089986375647232 |
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| author | Simeon, Fritz Tam, Michael K.C. Hatton, T. Alan Too, Heng-Phon |
| author_facet | Simeon, Fritz Tam, Michael K.C. Hatton, T. Alan Too, Heng-Phon |
| author_sort | Simeon, Fritz |
| collection | MIT |
| description | Development of safe and efficient synthetic gene delivery vectors is hampered with limited understanding the fundamental correlation of physicochemical properties of the vectors with their biological activities. Five major barriers contributing to poor transfection efficiency of synthetic vectors include cellular association, endosomal escape, intracellular trafficking, nuclear translocation and transcription of exogenous genes. In this study, the correlation of physicochemical properties of polymer-based synthetic gene delivery vectors (polyplexes) with cellular association as the first barrier for in vitro transfection was investigated. Polyethylene oxide block copolymer with poly(2-(dimethylamino)ethyl methacryate) (PEO-b-pDMAEMA) was chosen as the model in this study. Cellular association and transfection efficiency of block copolymer complexes were studied in Neuro2A cells. Quantitative real time polymerase chain reaction (PCR) was applied to elucidate the cellular association of polyplexes. Physicochemical properties of the vectors including size and surface charge were characterized using light scattering measurements. Formation of aggregate was found as the major indication for high cellular association and uptake for in vitro transfection. |
| first_indexed | 2024-09-23T14:29:05Z |
| format | Article |
| id | mit-1721.1/35871 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:29:05Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | mit-1721.1/358712019-04-10T09:58:51Z Aggregation of Synthetic Gene Delivery Vectors Enhance the Cellular Association and Uptake for in vitro Transfection Simeon, Fritz Tam, Michael K.C. Hatton, T. Alan Too, Heng-Phon PEO-b-pDMAEMA DLS Zeta Potential Transfection Quantitative Real Time PCR Development of safe and efficient synthetic gene delivery vectors is hampered with limited understanding the fundamental correlation of physicochemical properties of the vectors with their biological activities. Five major barriers contributing to poor transfection efficiency of synthetic vectors include cellular association, endosomal escape, intracellular trafficking, nuclear translocation and transcription of exogenous genes. In this study, the correlation of physicochemical properties of polymer-based synthetic gene delivery vectors (polyplexes) with cellular association as the first barrier for in vitro transfection was investigated. Polyethylene oxide block copolymer with poly(2-(dimethylamino)ethyl methacryate) (PEO-b-pDMAEMA) was chosen as the model in this study. Cellular association and transfection efficiency of block copolymer complexes were studied in Neuro2A cells. Quantitative real time polymerase chain reaction (PCR) was applied to elucidate the cellular association of polyplexes. Physicochemical properties of the vectors including size and surface charge were characterized using light scattering measurements. Formation of aggregate was found as the major indication for high cellular association and uptake for in vitro transfection. Singapore-MIT Alliance (SMA) 2007-02-05T23:51:52Z 2007-02-05T23:51:52Z 2007-01 Article http://hdl.handle.net/1721.1/35871 en Chemical and Pharmaceutical Engineering (CPE) application/pdf |
| spellingShingle | PEO-b-pDMAEMA DLS Zeta Potential Transfection Quantitative Real Time PCR Simeon, Fritz Tam, Michael K.C. Hatton, T. Alan Too, Heng-Phon Aggregation of Synthetic Gene Delivery Vectors Enhance the Cellular Association and Uptake for in vitro Transfection |
| title | Aggregation of Synthetic Gene Delivery Vectors Enhance the Cellular Association and Uptake for in vitro Transfection |
| title_full | Aggregation of Synthetic Gene Delivery Vectors Enhance the Cellular Association and Uptake for in vitro Transfection |
| title_fullStr | Aggregation of Synthetic Gene Delivery Vectors Enhance the Cellular Association and Uptake for in vitro Transfection |
| title_full_unstemmed | Aggregation of Synthetic Gene Delivery Vectors Enhance the Cellular Association and Uptake for in vitro Transfection |
| title_short | Aggregation of Synthetic Gene Delivery Vectors Enhance the Cellular Association and Uptake for in vitro Transfection |
| title_sort | aggregation of synthetic gene delivery vectors enhance the cellular association and uptake for in vitro transfection |
| topic | PEO-b-pDMAEMA DLS Zeta Potential Transfection Quantitative Real Time PCR |
| url | http://hdl.handle.net/1721.1/35871 |
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