Digital holographic imaging of microorganisms
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2006.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2007
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| Online Access: | http://hdl.handle.net/1721.1/36684 |
| _version_ | 1826214069538914304 |
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| author | Wolf, Michael Trevor |
| author2 | Roman Stocker. |
| author_facet | Roman Stocker. Wolf, Michael Trevor |
| author_sort | Wolf, Michael Trevor |
| collection | MIT |
| description | Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2006. |
| first_indexed | 2024-09-23T15:59:18Z |
| format | Thesis |
| id | mit-1721.1/36684 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T15:59:18Z |
| publishDate | 2007 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/366842019-04-10T15:21:18Z Digital holographic imaging of microorganisms DHI of microorganisms Wolf, Michael Trevor Roman Stocker. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Mechanical Engineering. Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2006. Includes bibliographical references (leaf 25). Imaging aquatic microorganisms in 3D space is of interest to biologists and ocean scientists seeking to understand the behavior of these organisms in their natural environments. In this research, digital holographic imaging (DHI), with a 4f system providing transverse magnification of 9.1, is used to study such microorganisms. To test the imaging technique, DHI was used to locate and track 10 micrometer Dunaliella freely swimming in a 30 milliliter tank of artificial ocean water. Multiple holograms were recorded onto one frame with laser pulsing to identify short algae trajectories. An automatic algae locating program was designed, but the signal to noise ratio was too low, and therefore the program could only locate algae reliably with manual confirmation. With refinement to the experimental setup, the signal to noise ratio could be increased, and this imaging technique could be used to analyze many systems of aquatic microorganisms interacting in a 3D space. by Michael Trevor Wolf. S.B. 2007-03-12T17:44:09Z 2007-03-12T17:44:09Z 2006 2006 Thesis http://hdl.handle.net/1721.1/36684 77536963 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 25 leaves application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Wolf, Michael Trevor Digital holographic imaging of microorganisms |
| title | Digital holographic imaging of microorganisms |
| title_full | Digital holographic imaging of microorganisms |
| title_fullStr | Digital holographic imaging of microorganisms |
| title_full_unstemmed | Digital holographic imaging of microorganisms |
| title_short | Digital holographic imaging of microorganisms |
| title_sort | digital holographic imaging of microorganisms |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/36684 |
| work_keys_str_mv | AT wolfmichaeltrevor digitalholographicimagingofmicroorganisms AT wolfmichaeltrevor dhiofmicroorganisms |