Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2006.
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| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2007
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| Online Access: | http://hdl.handle.net/1721.1/36685 |
| _version_ | 1826195068069871616 |
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| author | Was, Adam (Adam S.) |
| author2 | Richard T. Lee. |
| author_facet | Richard T. Lee. Was, Adam (Adam S.) |
| author_sort | Was, Adam (Adam S.) |
| collection | MIT |
| description | Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2006. |
| first_indexed | 2024-09-23T10:06:49Z |
| format | Thesis |
| id | mit-1721.1/36685 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T10:06:49Z |
| publishDate | 2007 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/366852019-04-11T09:04:11Z Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery Novel recombinant protein constructs for improved IGF-1 delivery Was, Adam (Adam S.) Richard T. Lee. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Mechanical Engineering. Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2006. Includes bibliographical references (p. 87-89). Four novel recombinant protein constructs were designed to improve the delivery of insulin-like growth factor-1 (IGF-1). The IGF-1 sequence was subcloned into a HisMax plasmid and expressed at low levels by transient transfection in 293 cells. Site-specific mutagenesis was used to insert all four construct sequences into the IGF-1 HisMax plasmid, and to insert a secretory sequence into all four construct plasmids. A stable cell line selection in 293 and CHO cells using Zeocin was attempted. The four construct sequences were then subcloned from the HisMax plasmids to TrcHis plasmids and expressed in bacteria for higher production efficiency. Ni-NTA purified protein was detected in three of the four constructs. These proteins may ultimately be useful for myocardial delivery of IGF-1 to facilitate cardiac repair. by Adam Was. S.B. 2007-03-12T17:44:16Z 2007-03-12T17:44:16Z 2006 2006 Thesis http://hdl.handle.net/1721.1/36685 77536995 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 89 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Was, Adam (Adam S.) Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery |
| title | Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery |
| title_full | Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery |
| title_fullStr | Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery |
| title_full_unstemmed | Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery |
| title_short | Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery |
| title_sort | novel recombinant protein constructs for improved insulin like growth factor 1 delivery |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/36685 |
| work_keys_str_mv | AT wasadamadams novelrecombinantproteinconstructsforimprovedinsulinlikegrowthfactor1delivery AT wasadamadams novelrecombinantproteinconstructsforimprovedigf1delivery |