Online Raman spectroscopy for bioprocess monitoring
Thesis (M. Eng. and S.B.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2005.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2007
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| Online Access: | http://hdl.handle.net/1721.1/36757 |
| _version_ | 1811088734407360512 |
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| author | Gil, Gustavo Adolfo |
| author2 | Rajeev Ram. |
| author_facet | Rajeev Ram. Gil, Gustavo Adolfo |
| author_sort | Gil, Gustavo Adolfo |
| collection | MIT |
| description | Thesis (M. Eng. and S.B.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2005. |
| first_indexed | 2024-09-23T14:06:42Z |
| format | Thesis |
| id | mit-1721.1/36757 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T14:06:42Z |
| publishDate | 2007 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/367572019-04-12T15:59:16Z Online Raman spectroscopy for bioprocess monitoring Gil, Gustavo Adolfo Rajeev Ram. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Electrical Engineering and Computer Science. Thesis (M. Eng. and S.B.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2005. Author received the S.B. degree, June 2005 and the M. Eng. degree, Sept. 2005. Includes bibliographical references (p. 195-202). Online monitoring of bioprocesses is essential to expanding the potential of biotechnology. In this thesis, a system to estimate concentrations of chemical components of an Escherichia Coli fermentation growth medium via a remote fiber-optic Raman spectroscopy probe was studied in depth. The system was characterized to determine sources of instability and systematic error. A complete first-order error analysis was conducted to determine the theoretical sensitivity of the instrument. A suite of improvements and new features, including an online estimation of optical density and biomass, a method to correct for wavelength shifts, and a setup to increase repeatability and throughput for offline and calibration methods was developed accordingly. The theoretical and experimental ground work for developing a correction for spectrum distortions caused by elastic scattering, a fundamental problem for many spectroscopic applications, was laid out. In addition, offline Raman spectroscopy was used to estimate concentrations of fructose, glucose, sucrose, and nitrate in an oil palm (Elais guineensis) bioreaction. Finally, an expansion of optical techniques into new scale-up applications in plant cell bioprocesses, such as plant call differentiation was explored. by Gustavo Adolfo Gil. M.Eng.and S.B. 2007-03-12T17:50:56Z 2007-03-12T17:50:56Z 2005 2005 Thesis http://hdl.handle.net/1721.1/36757 78616168 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 202 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Electrical Engineering and Computer Science. Gil, Gustavo Adolfo Online Raman spectroscopy for bioprocess monitoring |
| title | Online Raman spectroscopy for bioprocess monitoring |
| title_full | Online Raman spectroscopy for bioprocess monitoring |
| title_fullStr | Online Raman spectroscopy for bioprocess monitoring |
| title_full_unstemmed | Online Raman spectroscopy for bioprocess monitoring |
| title_short | Online Raman spectroscopy for bioprocess monitoring |
| title_sort | online raman spectroscopy for bioprocess monitoring |
| topic | Electrical Engineering and Computer Science. |
| url | http://hdl.handle.net/1721.1/36757 |
| work_keys_str_mv | AT gilgustavoadolfo onlineramanspectroscopyforbioprocessmonitoring |