Volumetric reconstruction of tissue structure from two-dimensional microscopy images

Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, June 2006.

Bibliographic Details
Main Author: Cruz, Francisco (Francisco Ui)
Other Authors: Polina Golland and Richard T. Lee.
Format: Thesis
Language:eng
Published: Massachusetts Institute of Technology 2007
Subjects:
Online Access:http://hdl.handle.net/1721.1/37051
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author Cruz, Francisco (Francisco Ui)
author2 Polina Golland and Richard T. Lee.
author_facet Polina Golland and Richard T. Lee.
Cruz, Francisco (Francisco Ui)
author_sort Cruz, Francisco (Francisco Ui)
collection MIT
description Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, June 2006.
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spelling mit-1721.1/370512019-04-10T18:17:58Z Volumetric reconstruction of tissue structure from two-dimensional microscopy images Cruz, Francisco (Francisco Ui) Polina Golland and Richard T. Lee. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Electrical Engineering and Computer Science. Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, June 2006. Includes bibliographical references (leaf 41). Cell morphology of tissue is naturally three-dimensional. Most current methods for tissue analysis use two dimensional histological images of the tissue samples, restricting the analysis to 2D. Existing approaches do not provide essential three-dimensional information such as cell volume, shape and structural orientation of cells within the tissue. This thesis investigates a method to extract three dimensional data using two-dimensional microscopy. We demonstrate that three dimensional cell structure can be acquired using two dimensional fluorescence microscopy and two-photon microscopy and explore the application of the analysis to studies of cardiac tissue. by Francisco Cruz. M.Eng. 2007-04-03T17:05:58Z 2007-04-03T17:05:58Z 2005 2006 Thesis http://hdl.handle.net/1721.1/37051 79476436 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 55 leaves application/pdf Massachusetts Institute of Technology
spellingShingle Electrical Engineering and Computer Science.
Cruz, Francisco (Francisco Ui)
Volumetric reconstruction of tissue structure from two-dimensional microscopy images
title Volumetric reconstruction of tissue structure from two-dimensional microscopy images
title_full Volumetric reconstruction of tissue structure from two-dimensional microscopy images
title_fullStr Volumetric reconstruction of tissue structure from two-dimensional microscopy images
title_full_unstemmed Volumetric reconstruction of tissue structure from two-dimensional microscopy images
title_short Volumetric reconstruction of tissue structure from two-dimensional microscopy images
title_sort volumetric reconstruction of tissue structure from two dimensional microscopy images
topic Electrical Engineering and Computer Science.
url http://hdl.handle.net/1721.1/37051
work_keys_str_mv AT cruzfranciscofranciscoui volumetricreconstructionoftissuestructurefromtwodimensionalmicroscopyimages