Stress and release : chemical modulation of secondary metabolite production in Aspergillus sp.
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2006.
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| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2007
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| Online Access: | http://hdl.handle.net/1721.1/37692 |
| _version_ | 1811073382545883136 |
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| author | Hanlon, Amy |
| author2 | Sarah E. O'Connor. |
| author_facet | Sarah E. O'Connor. Hanlon, Amy |
| author_sort | Hanlon, Amy |
| collection | MIT |
| description | Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2006. |
| first_indexed | 2024-09-23T09:32:12Z |
| format | Thesis |
| id | mit-1721.1/37692 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T09:32:12Z |
| publishDate | 2007 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/376922019-04-10T21:13:26Z Stress and release : chemical modulation of secondary metabolite production in Aspergillus sp. Hanlon, Amy Sarah E. O'Connor. Massachusetts Institute of Technology. Dept. of Chemistry. Massachusetts Institute of Technology. Dept. of Chemistry. Chemistry. Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2006. Includes bibliographical references (leaf 18). Cyclosporin A induced biosynthesis of colored compounds in three species of Aspergillus. Diode array HPLC MS analysis of culture extracts revealed Aspergillus terreus demonstrated the most profound response, with upregulation of more then twelve compounds from three distinct chemical families; butyrolactones, aspulvinones, and asterriquinones. Compounds from these three families are prenylated, and biosynthetically derived from homodimers of amino acids. The majority of the upregulated compounds were the aspulvinones, a class of butenolides. Structural elucidation of four isolated aspulvinones revealed both known and novel structures. Inducer concentration had a significant impact on aspulvinone profile. Bioassay revealed previously unreported antibacterial activity for the aspulvinones. A high-throughput colorimetric screen was designed to probe the response. The screen of 2480 known bioactives revealed multiple compounds capable of inducing aspulvinone production, and one compound, rapamycin, capable of inhibiting the response. Additionally, the increase in aspulvinone production was correlated with a decrease in culture density, indicating aspulvinone production is a general stress response. by Amy Hanlon. S.M. 2007-06-28T12:24:20Z 2007-06-28T12:24:20Z 2006 2006 Thesis http://hdl.handle.net/1721.1/37692 129988674 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 18 leaves application/pdf Massachusetts Institute of Technology |
| spellingShingle | Chemistry. Hanlon, Amy Stress and release : chemical modulation of secondary metabolite production in Aspergillus sp. |
| title | Stress and release : chemical modulation of secondary metabolite production in Aspergillus sp. |
| title_full | Stress and release : chemical modulation of secondary metabolite production in Aspergillus sp. |
| title_fullStr | Stress and release : chemical modulation of secondary metabolite production in Aspergillus sp. |
| title_full_unstemmed | Stress and release : chemical modulation of secondary metabolite production in Aspergillus sp. |
| title_short | Stress and release : chemical modulation of secondary metabolite production in Aspergillus sp. |
| title_sort | stress and release chemical modulation of secondary metabolite production in aspergillus sp |
| topic | Chemistry. |
| url | http://hdl.handle.net/1721.1/37692 |
| work_keys_str_mv | AT hanlonamy stressandreleasechemicalmodulationofsecondarymetaboliteproductioninaspergillussp |