Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture
Glycosylation of recombinant proteins can change during the culture of animal cells. Since lipid-linked oligosaccharides (LLOs) are the carbohydrate donors in N-linked glycosylation, their availability is postulated to influence the extent of glycosylation. To test this hypothesis, relative LLO an...
| Main Authors: | , |
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| Format: | Article |
| Language: | en_US |
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2003
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| Online Access: | http://hdl.handle.net/1721.1/3776 |
| _version_ | 1826203482006224896 |
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| author | Yuk, Inn Huam Yvonne. Wang, Daniel I.C. |
| author_facet | Yuk, Inn Huam Yvonne. Wang, Daniel I.C. |
| author_sort | Yuk, Inn Huam Yvonne. |
| collection | MIT |
| description | Glycosylation of recombinant proteins can change during the culture of animal cells. Since lipid-linked oligosaccharides (LLOs) are the carbohydrate donors in N-linked glycosylation, their availability is postulated to influence the extent of glycosylation. To test this hypothesis, relative LLO and glycosylation levels in Chinese hamster ovary (CHO) cells were monitored over the course of batch culture for corresponding changes. Radiolabelling studies reveal that throughout the length of culture, intracellular LLO levels remained within a 2-fold range and overall CHO protein glycosylation varied by less than 30%. The implications of these results and hypotheses to explain the findings are discussed. |
| first_indexed | 2024-09-23T12:37:43Z |
| format | Article |
| id | mit-1721.1/3776 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:37:43Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | mit-1721.1/37762019-04-11T02:52:28Z Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture Yuk, Inn Huam Yvonne. Wang, Daniel I.C. G0G1 phase lipid-linked oligosaccharide Glycosylation of recombinant proteins can change during the culture of animal cells. Since lipid-linked oligosaccharides (LLOs) are the carbohydrate donors in N-linked glycosylation, their availability is postulated to influence the extent of glycosylation. To test this hypothesis, relative LLO and glycosylation levels in Chinese hamster ovary (CHO) cells were monitored over the course of batch culture for corresponding changes. Radiolabelling studies reveal that throughout the length of culture, intracellular LLO levels remained within a 2-fold range and overall CHO protein glycosylation varied by less than 30%. The implications of these results and hypotheses to explain the findings are discussed. Singapore-MIT Alliance (SMA) 2003-12-08T15:03:31Z 2003-12-08T15:03:31Z 2003-01 Article http://hdl.handle.net/1721.1/3776 en_US Molecular Engineering of Biological and Chemical Systems (MEBCS); 11281124 bytes application/pdf application/pdf |
| spellingShingle | G0G1 phase lipid-linked oligosaccharide Yuk, Inn Huam Yvonne. Wang, Daniel I.C. Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture |
| title | Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture |
| title_full | Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture |
| title_fullStr | Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture |
| title_full_unstemmed | Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture |
| title_short | Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture |
| title_sort | changes in the overall extent of protein glycosylation by chinese hamster ovary cells over the course of batch culture |
| topic | G0G1 phase lipid-linked oligosaccharide |
| url | http://hdl.handle.net/1721.1/3776 |
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