Engineering Mammalian Cells for Improved Recombinant Protein Production
The production of recombinant glycoproteins from mammalian cell cultures requires robust processes that can achieve high protein yield while ensuring the efficacy of these proteins as human therapeutics. We describe two approaches currently being developed in our group to genetically engineer cell l...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | en_US |
| Published: |
2003
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/1721.1/3781 |
| _version_ | 1826193676750028800 |
|---|---|
| author | Wong, Niki S.C. Tan, Hong-Kiat Wang, Daniel I.C. Yap, Miranda G.S. |
| author_facet | Wong, Niki S.C. Tan, Hong-Kiat Wang, Daniel I.C. Yap, Miranda G.S. |
| author_sort | Wong, Niki S.C. |
| collection | MIT |
| description | The production of recombinant glycoproteins from mammalian cell cultures requires robust processes that can achieve high protein yield while ensuring the efficacy of these proteins as human therapeutics. We describe two approaches currently being developed in our group to genetically engineer cell lines with desirable characteristics for recombinant protein production. To enhance the degree of sialylation in the glycoprotein product, we propose to increase intracellular sialic acid availability by overexpressing the CMP-sialic acid transporters. We are also interested in engineering mammalian cells that can proliferate at reduced cultivation temperatures. Low temperature cultivation of mammalian cells has been shown to enhance glycoprotein production but reduces cell growth. It is hypothesized that a mutant cell line that can proliferate at low temperatures may be coupled with low temperature cultivation to improve recombinant protein production. |
| first_indexed | 2024-09-23T09:42:56Z |
| format | Article |
| id | mit-1721.1/3781 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:42:56Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | mit-1721.1/37812019-04-10T16:32:12Z Engineering Mammalian Cells for Improved Recombinant Protein Production Wong, Niki S.C. Tan, Hong-Kiat Wang, Daniel I.C. Yap, Miranda G.S. recombinant glycoproteins recombinant protein production sialylation CMP-sialic acid transporters The production of recombinant glycoproteins from mammalian cell cultures requires robust processes that can achieve high protein yield while ensuring the efficacy of these proteins as human therapeutics. We describe two approaches currently being developed in our group to genetically engineer cell lines with desirable characteristics for recombinant protein production. To enhance the degree of sialylation in the glycoprotein product, we propose to increase intracellular sialic acid availability by overexpressing the CMP-sialic acid transporters. We are also interested in engineering mammalian cells that can proliferate at reduced cultivation temperatures. Low temperature cultivation of mammalian cells has been shown to enhance glycoprotein production but reduces cell growth. It is hypothesized that a mutant cell line that can proliferate at low temperatures may be coupled with low temperature cultivation to improve recombinant protein production. Singapore-MIT Alliance (SMA) 2003-12-08T15:17:15Z 2003-12-08T15:17:15Z 2003-01 Article http://hdl.handle.net/1721.1/3781 en_US Molecular Engineering of Biological and Chemical Systems (MEBCS); 11114 bytes application/pdf application/pdf |
| spellingShingle | recombinant glycoproteins recombinant protein production sialylation CMP-sialic acid transporters Wong, Niki S.C. Tan, Hong-Kiat Wang, Daniel I.C. Yap, Miranda G.S. Engineering Mammalian Cells for Improved Recombinant Protein Production |
| title | Engineering Mammalian Cells for Improved Recombinant Protein Production |
| title_full | Engineering Mammalian Cells for Improved Recombinant Protein Production |
| title_fullStr | Engineering Mammalian Cells for Improved Recombinant Protein Production |
| title_full_unstemmed | Engineering Mammalian Cells for Improved Recombinant Protein Production |
| title_short | Engineering Mammalian Cells for Improved Recombinant Protein Production |
| title_sort | engineering mammalian cells for improved recombinant protein production |
| topic | recombinant glycoproteins recombinant protein production sialylation CMP-sialic acid transporters |
| url | http://hdl.handle.net/1721.1/3781 |
| work_keys_str_mv | AT wongnikisc engineeringmammaliancellsforimprovedrecombinantproteinproduction AT tanhongkiat engineeringmammaliancellsforimprovedrecombinantproteinproduction AT wangdanielic engineeringmammaliancellsforimprovedrecombinantproteinproduction AT yapmirandags engineeringmammaliancellsforimprovedrecombinantproteinproduction |