Quantification and signaling of alternatively spliced GFRα2 isoforms
Neurturin (NTN) belongs to the glial cell-line derived neurotrophic factor (GDNF) family of growth factors. Both NTN and GDNF have been shown to potently prevent the degeneration of dopaminergic neuron in vitro and in vivo. The GDNF family receptor alpha 2 (GFRα-2) is the preferred receptor for NTN....
| Main Authors: | , |
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| Format: | Article |
| Language: | en_US |
| Published: |
2003
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| Online Access: | http://hdl.handle.net/1721.1/3789 |
| _version_ | 1826208505095258112 |
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| author | Too, Heng-Phon Fung, Winnie Kar Yee |
| author_facet | Too, Heng-Phon Fung, Winnie Kar Yee |
| author_sort | Too, Heng-Phon |
| collection | MIT |
| description | Neurturin (NTN) belongs to the glial cell-line derived neurotrophic factor (GDNF) family of growth factors. Both NTN and GDNF have been shown to potently prevent the degeneration of dopaminergic neuron in vitro and in vivo. The GDNF family receptor alpha 2 (GFRα-2) is the preferred receptor for NTN. In addition to the known full-length isoform (GFRα-2a), we have previously reported the isolation of two novel alternatively spliced isoforms (GFRα-2b and GFRα-2c). The expression levels of these isoforms have yet to be quantified and the functional properties determined. In this report, we have developed a real time polymerase chain reaction (PCR) using SYBR Green I to detect the expression levels of the three splice variants (GFRα-2a, GFRα-2b and GFRα-2c) in murine tissues. Both GFRα-2a and GFRα-2c were expressed at similar levels in all tissues examined. GFRα-2b was found to be 10 fold lower in expression. All three isoforms activated MAPK (ERK1/2) and Akt. Transcriptional profiling with DNA microarrays demonstrated that the spliced isoforms do not share similar profiles. In conclusion, we have now shown the expression levels of the spliced variants. All three isoforms are functional. However, each isoform appeared to have unique transcriptional profiles when activated. |
| first_indexed | 2024-09-23T14:06:24Z |
| format | Article |
| id | mit-1721.1/3789 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:06:24Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | mit-1721.1/37892019-04-12T08:06:44Z Quantification and signaling of alternatively spliced GFRα2 isoforms Too, Heng-Phon Fung, Winnie Kar Yee GDNF quantitative real time PCR GFRα-2 Neurturin (NTN) belongs to the glial cell-line derived neurotrophic factor (GDNF) family of growth factors. Both NTN and GDNF have been shown to potently prevent the degeneration of dopaminergic neuron in vitro and in vivo. The GDNF family receptor alpha 2 (GFRα-2) is the preferred receptor for NTN. In addition to the known full-length isoform (GFRα-2a), we have previously reported the isolation of two novel alternatively spliced isoforms (GFRα-2b and GFRα-2c). The expression levels of these isoforms have yet to be quantified and the functional properties determined. In this report, we have developed a real time polymerase chain reaction (PCR) using SYBR Green I to detect the expression levels of the three splice variants (GFRα-2a, GFRα-2b and GFRα-2c) in murine tissues. Both GFRα-2a and GFRα-2c were expressed at similar levels in all tissues examined. GFRα-2b was found to be 10 fold lower in expression. All three isoforms activated MAPK (ERK1/2) and Akt. Transcriptional profiling with DNA microarrays demonstrated that the spliced isoforms do not share similar profiles. In conclusion, we have now shown the expression levels of the spliced variants. All three isoforms are functional. However, each isoform appeared to have unique transcriptional profiles when activated. Singapore-MIT Alliance (SMA) 2003-12-08T15:57:49Z 2003-12-08T15:57:49Z 2003-01 Article http://hdl.handle.net/1721.1/3789 en_US Molecular Engineering of Biological and Chemical Systems (MEBCS); 330964 bytes application/pdf application/pdf |
| spellingShingle | GDNF quantitative real time PCR GFRα-2 Too, Heng-Phon Fung, Winnie Kar Yee Quantification and signaling of alternatively spliced GFRα2 isoforms |
| title | Quantification and signaling of alternatively spliced GFRα2 isoforms |
| title_full | Quantification and signaling of alternatively spliced GFRα2 isoforms |
| title_fullStr | Quantification and signaling of alternatively spliced GFRα2 isoforms |
| title_full_unstemmed | Quantification and signaling of alternatively spliced GFRα2 isoforms |
| title_short | Quantification and signaling of alternatively spliced GFRα2 isoforms |
| title_sort | quantification and signaling of alternatively spliced gfrα2 isoforms |
| topic | GDNF quantitative real time PCR GFRα-2 |
| url | http://hdl.handle.net/1721.1/3789 |
| work_keys_str_mv | AT toohengphon quantificationandsignalingofalternativelysplicedgfra2isoforms AT fungwinniekaryee quantificationandsignalingofalternativelysplicedgfra2isoforms |