Investigating the role of calcium in the biomechanical response of neutrophils to mechanical deformation experienced in the pulmonary capillaries
Thesis (M. Eng.)--Massachusetts Institute of Technology, Biological Engineering Division, 2006.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2007
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| Online Access: | http://hdl.handle.net/1721.1/37960 |
| _version_ | 1811092383761170432 |
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| author | Hsu, Jeffrey J |
| author2 | Massachusetts Institute of Technology. Biological Engineering Division. |
| author_facet | Massachusetts Institute of Technology. Biological Engineering Division. Hsu, Jeffrey J |
| author_sort | Hsu, Jeffrey J |
| collection | MIT |
| description | Thesis (M. Eng.)--Massachusetts Institute of Technology, Biological Engineering Division, 2006. |
| first_indexed | 2024-09-23T15:17:18Z |
| format | Thesis |
| id | mit-1721.1/37960 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T15:17:18Z |
| publishDate | 2007 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/379602019-04-12T07:29:53Z Investigating the role of calcium in the biomechanical response of neutrophils to mechanical deformation experienced in the pulmonary capillaries Hsu, Jeffrey J Massachusetts Institute of Technology. Biological Engineering Division. Massachusetts Institute of Technology. Biological Engineering Division. Biological Engineering Division. Thesis (M. Eng.)--Massachusetts Institute of Technology, Biological Engineering Division, 2006. Includes bibliographical references (p. 73-79). Neutrophils in the pulmonary microcirculation are subjected to mechanical deformation while traveling through capillaries of sizes much smaller than the mean neutrophil diameter. This deformation has been shown to result in significant reductions in both the shear storage and shear loss moduli of the cell, with subsequent recovery towards their initial values. Also, deformation above a threshold stimulus results in neutrophil activation, evidenced by pseudopod projection from the cell. These two events are thought to occur via independent pathways, yet little is known about the mechanosensing signaling involved. Other work has demonstrated that physiological deformation of neutrophils induces a marked increase in the levels of cytosolic calcium, suggesting that this occurrence may trigger the biomechanical response observed in the cell. The aim of this thesis was to elucidate the role of calcium in the neutrophil response to the mechanical deformation experienced during transit through the pulmonary capillaries. (cont.) Chelating intracellular calcium in neutrophils resulted in (i) decreased deformability of the cells into a microchannel, (ii) attenuation of the drop in shear storage modulus (G') observed in untreated cells upon deformation, and (iii) shorter activation times. These findings suggest that cytosolic calcium holds an important function in the neutrophil transit through the capillaries, and inhibition of normal calcium release within the cell can lead to leukostasis-like conditions. by Jeffrey J Hsu. M.Eng. 2007-07-18T13:16:34Z 2007-07-18T13:16:34Z 2006 2006 Thesis http://hdl.handle.net/1721.1/37960 144608620 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 122 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biological Engineering Division. Hsu, Jeffrey J Investigating the role of calcium in the biomechanical response of neutrophils to mechanical deformation experienced in the pulmonary capillaries |
| title | Investigating the role of calcium in the biomechanical response of neutrophils to mechanical deformation experienced in the pulmonary capillaries |
| title_full | Investigating the role of calcium in the biomechanical response of neutrophils to mechanical deformation experienced in the pulmonary capillaries |
| title_fullStr | Investigating the role of calcium in the biomechanical response of neutrophils to mechanical deformation experienced in the pulmonary capillaries |
| title_full_unstemmed | Investigating the role of calcium in the biomechanical response of neutrophils to mechanical deformation experienced in the pulmonary capillaries |
| title_short | Investigating the role of calcium in the biomechanical response of neutrophils to mechanical deformation experienced in the pulmonary capillaries |
| title_sort | investigating the role of calcium in the biomechanical response of neutrophils to mechanical deformation experienced in the pulmonary capillaries |
| topic | Biological Engineering Division. |
| url | http://hdl.handle.net/1721.1/37960 |
| work_keys_str_mv | AT hsujeffreyj investigatingtheroleofcalciuminthebiomechanicalresponseofneutrophilstomechanicaldeformationexperiencedinthepulmonarycapillaries |