An apparatus for high throughput muscle cell experimentation
Thesis (Ph. D.)--Massachusetts Institute of Technology, Biological Engineering Division, 2006.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2007
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| Online Access: | http://hdl.handle.net/1721.1/38240 |
| _version_ | 1826217231899426816 |
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| author | Garcia-Webb, Michael G. (Michael Gregory) |
| author2 | Ian W. Hunter. |
| author_facet | Ian W. Hunter. Garcia-Webb, Michael G. (Michael Gregory) |
| author_sort | Garcia-Webb, Michael G. (Michael Gregory) |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Biological Engineering Division, 2006. |
| first_indexed | 2024-09-23T17:00:04Z |
| format | Thesis |
| id | mit-1721.1/38240 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T17:00:04Z |
| publishDate | 2007 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/382402019-05-22T03:10:42Z An apparatus for high throughput muscle cell experimentation Garcia-Webb, Michael G. (Michael Gregory) Ian W. Hunter. Massachusetts Institute of Technology. Biological Engineering Division. Massachusetts Institute of Technology. Biological Engineering Division. Biological Engineering Division. Thesis (Ph. D.)--Massachusetts Institute of Technology, Biological Engineering Division, 2006. MIT Science Library copy: printed in pages versus leaves. Also issued in pages. Includes bibliographical references (leaves 183-197). The cardiac ventricular muscle cell (myocyte) is a key experimental system for exploring the mechanical properties of the diseased and healthy heart. The myocyte experimental model provides a higher level of physiological relevance than molecular or myofibril studies while avoiding problems inherent to multicellular preparations including heterogeneity of cell types and diffusion limited extracellular spaces. Millions of primary myocytes that remain viable for four to six hours can be readily isolated from animal models. However, the mechanical properties of only a few physically loaded myocytes can be explored in this time period using current, bulky and expensive instrumentation. In this thesis, a prototype instrument is described that is modular and inexpensive and could form the basis of an array of devices for probing the mechanical properties of single mammnalian myocytes in parallel. This would greatly increase the throughput of scientific experimentation and could be applied as a high content screening instrument in the pharmaceutical industry providing information at the level of a critical cellular phenotype, myocyte mechanical properties, for drug development and toxicology studies. (cont.) The design, development and experimental verification of the modular instrument are presented here. The mathematical, mechanical and electrical characteristics of the novel force sensor and actuator system, Ho control implementation and data processing methodology are discussed. Finally, the functionality of the instrument is demonstrated by implementing novel methodologies for loading and attaching healthy, single mammalian ventricular myocytes to the force sensor and actuator and measuring their isometric twitch force and passive dynamic stiffness at varied sarcomere lengths. by Michael G. Garcia-Webb. Ph.D. 2007-08-03T18:18:11Z 2007-08-03T18:18:11Z 2006 2006 Thesis http://hdl.handle.net/1721.1/38240 146256772 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 204 leaves application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biological Engineering Division. Garcia-Webb, Michael G. (Michael Gregory) An apparatus for high throughput muscle cell experimentation |
| title | An apparatus for high throughput muscle cell experimentation |
| title_full | An apparatus for high throughput muscle cell experimentation |
| title_fullStr | An apparatus for high throughput muscle cell experimentation |
| title_full_unstemmed | An apparatus for high throughput muscle cell experimentation |
| title_short | An apparatus for high throughput muscle cell experimentation |
| title_sort | apparatus for high throughput muscle cell experimentation |
| topic | Biological Engineering Division. |
| url | http://hdl.handle.net/1721.1/38240 |
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