Eggshell Matrix Protein Mimetics: Elucidation of Molecular Mechanism of Goose Eggshell Calcification using Designed Peptides
Model peptides were designed, synthesized and conducted a detailed structure-property study to unravel the molecular mechanism of goose eggshell calcification. The peptides were designed based on the primary structural features of the eggshell matrix proteins ansocalcin and OC-17. In vitro CaCO₃ cry...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | en_US |
| Published: |
2003
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/1721.1/3929 |
| _version_ | 1811087485473652736 |
|---|---|
| author | Ajikumar, Parayil Kumaran Lakshminarayanan, Rajamani Valiyaveettil, Suresh Kini, R. Manjunatha |
| author_facet | Ajikumar, Parayil Kumaran Lakshminarayanan, Rajamani Valiyaveettil, Suresh Kini, R. Manjunatha |
| author_sort | Ajikumar, Parayil Kumaran |
| collection | MIT |
| description | Model peptides were designed, synthesized and conducted a detailed structure-property study to unravel the molecular mechanism of goose eggshell calcification. The peptides were designed based on the primary structural features of the eggshell matrix proteins ansocalcin and OC-17. In vitro CaCO₃ crystal growth experiments in presence of these peptides showed calcite crystal aggregation as observed in the case of the parent protein ansocalcin. The structure of these peptides in solution was established using intrinsic tryptophan fluorescence studies and quasi-elastic light scattering experiments. The structural features are correlated with observed results of the in vitro crystallization studies. |
| first_indexed | 2024-09-23T13:46:50Z |
| format | Article |
| id | mit-1721.1/3929 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:46:50Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | mit-1721.1/39292019-04-10T18:40:00Z Eggshell Matrix Protein Mimetics: Elucidation of Molecular Mechanism of Goose Eggshell Calcification using Designed Peptides Ajikumar, Parayil Kumaran Lakshminarayanan, Rajamani Valiyaveettil, Suresh Kini, R. Manjunatha ansocalcin biomineralization calcium carbonate designed peptides Model peptides were designed, synthesized and conducted a detailed structure-property study to unravel the molecular mechanism of goose eggshell calcification. The peptides were designed based on the primary structural features of the eggshell matrix proteins ansocalcin and OC-17. In vitro CaCO₃ crystal growth experiments in presence of these peptides showed calcite crystal aggregation as observed in the case of the parent protein ansocalcin. The structure of these peptides in solution was established using intrinsic tryptophan fluorescence studies and quasi-elastic light scattering experiments. The structural features are correlated with observed results of the in vitro crystallization studies. Singapore-MIT Alliance (SMA) 2003-12-16T02:43:43Z 2003-12-16T02:43:43Z 2004-01 Article http://hdl.handle.net/1721.1/3929 en_US Molecular Engineering of Biological and Chemical Systems (MEBCS); 262859 bytes application/pdf application/pdf |
| spellingShingle | ansocalcin biomineralization calcium carbonate designed peptides Ajikumar, Parayil Kumaran Lakshminarayanan, Rajamani Valiyaveettil, Suresh Kini, R. Manjunatha Eggshell Matrix Protein Mimetics: Elucidation of Molecular Mechanism of Goose Eggshell Calcification using Designed Peptides |
| title | Eggshell Matrix Protein Mimetics: Elucidation of Molecular Mechanism of Goose Eggshell Calcification using Designed Peptides |
| title_full | Eggshell Matrix Protein Mimetics: Elucidation of Molecular Mechanism of Goose Eggshell Calcification using Designed Peptides |
| title_fullStr | Eggshell Matrix Protein Mimetics: Elucidation of Molecular Mechanism of Goose Eggshell Calcification using Designed Peptides |
| title_full_unstemmed | Eggshell Matrix Protein Mimetics: Elucidation of Molecular Mechanism of Goose Eggshell Calcification using Designed Peptides |
| title_short | Eggshell Matrix Protein Mimetics: Elucidation of Molecular Mechanism of Goose Eggshell Calcification using Designed Peptides |
| title_sort | eggshell matrix protein mimetics elucidation of molecular mechanism of goose eggshell calcification using designed peptides |
| topic | ansocalcin biomineralization calcium carbonate designed peptides |
| url | http://hdl.handle.net/1721.1/3929 |
| work_keys_str_mv | AT ajikumarparayilkumaran eggshellmatrixproteinmimeticselucidationofmolecularmechanismofgooseeggshellcalcificationusingdesignedpeptides AT lakshminarayananrajamani eggshellmatrixproteinmimeticselucidationofmolecularmechanismofgooseeggshellcalcificationusingdesignedpeptides AT valiyaveettilsuresh eggshellmatrixproteinmimeticselucidationofmolecularmechanismofgooseeggshellcalcificationusingdesignedpeptides AT kinirmanjunatha eggshellmatrixproteinmimeticselucidationofmolecularmechanismofgooseeggshellcalcificationusingdesignedpeptides |