Pathogenesis of the carcinogenic bacterium, Helicobacter pylori
Thesis (Ph. D.)--Massachusetts Institute of Technology, Biological Engineering Division, 2007.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2009
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| Online Access: | http://dspace.mit.edu/handle/1721.1/39909 http://hdl.handle.net/1721.1/39909 |
| _version_ | 1826206130411405312 |
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| author | Lee, Chung-Wei, Ph. D. Massachusetts Institute of Technology |
| author2 | James G. Fox. |
| author_facet | James G. Fox. Lee, Chung-Wei, Ph. D. Massachusetts Institute of Technology |
| author_sort | Lee, Chung-Wei, Ph. D. Massachusetts Institute of Technology |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Biological Engineering Division, 2007. |
| first_indexed | 2024-09-23T13:24:34Z |
| format | Thesis |
| id | mit-1721.1/39909 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T13:24:34Z |
| publishDate | 2009 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/399092019-05-22T03:11:20Z Pathogenesis of the carcinogenic bacterium, Helicobacter pylori Lee, Chung-Wei, Ph. D. Massachusetts Institute of Technology James G. Fox. Massachusetts Institute of Technology. Biological Engineering Division. Massachusetts Institute of Technology. Biological Engineering Division. Biological Engineering Division. Thesis (Ph. D.)--Massachusetts Institute of Technology, Biological Engineering Division, 2007. Leaf 187 blank. Includes bibliographical references. Gastric cancer is the second most common malignancy in the digestive system and the second leading cause of cancer-related death worldwide. Epidemiological data and experimental studies have identified several risk factors for gastric cancer, including Helicobacter pylori infection, low fruit and vegetable intake, N-nitrosoamine exposure, high salt diet, and smoking. Among these risk factors, H. pylori infection is the major cause of gastric cancer. Therefore, H. pylori has been classified as a type 1 (definite) carcinogen for gastric cancer by the World Health Organization (WHO) in 1994. H. pylori colonizes the human stomach and has been definitively linked to chronic gastritis. Infection in some: susceptible individuals results in serious gastric disease such as peptic ulcer or gastric cancer. The first aim of this thesis was to examine the role of different T cell subpopulations in H. pylori gastritis. Using a murine adoptive transfer model, adoptive transfer of wildtype (wt) effector T cells (TE) into H. pylori-infected lymphopenic Rag2-/- recipient mice resulted in H. pylori-associated corpus gastritis superimposed with non-specific gastroduodenitis. Cotransfer with TE and regulatory T cells (TR) from wt or IL10-/- mice reduced gastroduodenitis, but only wt TR cells reduced corpus gastritis. (cont.) The second aim of this thesis was to evaluate the effect of vitamin C on H. pylori gastritis in vitamin C-deficient gulo-/- mice. It was found that a high vitamin C supplementation (3300 mg/L) in drinking water did not protect H. pylori gastritis, while a low vitamin C supplementation (33 mg/L) reduced the severity of H. pylori gastritis via an attenuated cellular immune response to H. pylori. The third aim of this thesis was to examine the role of DNA repair in H. pylori-associated gastric disease. We found that H. pylori-associated premalignant gastric atrophy was more severe in infected mice lacking DNA repair protein 3-alkyladenine DNA glycosylase or 06-methylguanine DNA methyltransferase in comparison to infected wt control mice. The forth aim of this thesis was to examine whether antimicrobial H. pylori eradication therapy could prevent gastric cancer development in INS-GAS mice, a model of gastric cancer. We found that antimicrobial H. pylori eradication therapy prevented the progression to gastric cancer in H. pylori-infected INS-GAS mice when treatment was instituted at an early stage of H. pylori infection. (cont.) In conclusion, these studies provide further insight into the role of host immune responses in H. pylori pathogenesis. Additionally, information was garnered regarding the roles of vitamin C supplementation, DNA repair proteins, and H. pylori eradication therapy in H. pylori-associated gastric disease using genetically manipulated mice. by Chung-Wei Lee. Ph.D. 2009-01-23T14:52:08Z 2009-01-23T14:52:08Z 2007 2007 Thesis http://dspace.mit.edu/handle/1721.1/39909 http://hdl.handle.net/1721.1/39909 182574790 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/39909 http://dspace.mit.edu/handle/1721.1/7582 187 leaves application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biological Engineering Division. Lee, Chung-Wei, Ph. D. Massachusetts Institute of Technology Pathogenesis of the carcinogenic bacterium, Helicobacter pylori |
| title | Pathogenesis of the carcinogenic bacterium, Helicobacter pylori |
| title_full | Pathogenesis of the carcinogenic bacterium, Helicobacter pylori |
| title_fullStr | Pathogenesis of the carcinogenic bacterium, Helicobacter pylori |
| title_full_unstemmed | Pathogenesis of the carcinogenic bacterium, Helicobacter pylori |
| title_short | Pathogenesis of the carcinogenic bacterium, Helicobacter pylori |
| title_sort | pathogenesis of the carcinogenic bacterium helicobacter pylori |
| topic | Biological Engineering Division. |
| url | http://dspace.mit.edu/handle/1721.1/39909 http://hdl.handle.net/1721.1/39909 |
| work_keys_str_mv | AT leechungweiphdmassachusettsinstituteoftechnology pathogenesisofthecarcinogenicbacteriumhelicobacterpylori |