An isoperibol calorimeter for the investigation of biochemical kinetics and isothermal titration calorimetry
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2008
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| Online Access: | http://hdl.handle.net/1721.1/40401 |
| _version_ | 1826199354812137472 |
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| author | Amadi, Ovid Charles |
| author2 | Ian W. Hunter. |
| author_facet | Ian W. Hunter. Amadi, Ovid Charles |
| author_sort | Amadi, Ovid Charles |
| collection | MIT |
| description | Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007. |
| first_indexed | 2024-09-23T11:18:53Z |
| format | Thesis |
| id | mit-1721.1/40401 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T11:18:53Z |
| publishDate | 2008 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/404012019-04-12T09:38:17Z An isoperibol calorimeter for the investigation of biochemical kinetics and isothermal titration calorimetry Amadi, Ovid Charles Ian W. Hunter. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Mechanical Engineering. Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007. Includes bibliographical references (leaf 52). Isothermal titration calorimetry is a technique used to measure the enthalpy change associated with a molecular binding interaction. From these data, the binding constant for the reaction can be determined. In the scope of a larger project to design a high sensitivity instrument for collecting such data, the current methods in isothermal titration calorimetry were investigated. Further calorimetric experience was acquired by designing a large scale calorimetric device. Dilution reactions with dimethyl sulfoxide and water were conducted to measure the excess enthalpy of binding. The inaccuracy of these measurements necessitated the more careful design of an isoperibol calorimeter. This calorimeter was modeled was an arrangement of coupled thermal masses and capacitances in order to fully understand its transient response to a thermal input. Dilution reactions and a neutralization reaction with HCl and NH40H were performed on the system and the results were used to make recommendations for the design of the future high sensitivity device. by Ovid Charles Amadi. S.B. 2008-02-27T22:21:29Z 2008-02-27T22:21:29Z 2007 2007 Thesis http://hdl.handle.net/1721.1/40401 191676863 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 52 leaves application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Amadi, Ovid Charles An isoperibol calorimeter for the investigation of biochemical kinetics and isothermal titration calorimetry |
| title | An isoperibol calorimeter for the investigation of biochemical kinetics and isothermal titration calorimetry |
| title_full | An isoperibol calorimeter for the investigation of biochemical kinetics and isothermal titration calorimetry |
| title_fullStr | An isoperibol calorimeter for the investigation of biochemical kinetics and isothermal titration calorimetry |
| title_full_unstemmed | An isoperibol calorimeter for the investigation of biochemical kinetics and isothermal titration calorimetry |
| title_short | An isoperibol calorimeter for the investigation of biochemical kinetics and isothermal titration calorimetry |
| title_sort | isoperibol calorimeter for the investigation of biochemical kinetics and isothermal titration calorimetry |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/40401 |
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