The effects of glycosaminoglycan content on the compressive modulus of chondrocyte seeded type II collagen scaffolds
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007.
| Main Author: | |
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2008
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| Online Access: | http://hdl.handle.net/1721.1/40463 |
| _version_ | 1811069277989502976 |
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| author | Pfeiffer, Emily (Emily R.) |
| author2 | Myron Spector. |
| author_facet | Myron Spector. Pfeiffer, Emily (Emily R.) |
| author_sort | Pfeiffer, Emily (Emily R.) |
| collection | MIT |
| description | Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007. |
| first_indexed | 2024-09-23T08:08:33Z |
| format | Thesis |
| id | mit-1721.1/40463 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T08:08:33Z |
| publishDate | 2008 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/404632019-04-09T16:53:49Z The effects of glycosaminoglycan content on the compressive modulus of chondrocyte seeded type II collagen scaffolds Effects of GAG content on the compressive chondrocyte seeded type II collagen scaffolds Pfeiffer, Emily (Emily R.) Myron Spector. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Mechanical Engineering. Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007. Includes bibliographical references (p. 34-36). This study examines glycosaminoglycan (GAG) density and aggregate compressive modulus HA of engineered cartilaginous implants. Culture parameters were developed to cause the goat articular chondrocyte seeded type II collagen scaffolds to generate 25 and 50% of the natural biochemical content of articular cartilage, with an overall goal of identifying construct compositions that might provide the most favorable response when implanted into defects in articular cartilage. Several scaffold cross-link densities were compared across constructs cultured in vitro to several time-points. The compressive modulus HA was measured through unconfined compression. One group of scaffolds averaged a compressive modulus one order of magnitude below that of natural tissue. Histological analysis verified that a chondrogenic phenotype was maintained and revealed a concentration of tissue development in the center of most scaffolds. This work includes a design for an original mechanical test apparatus for measuring the Poisson's ratio of the samples, enabling meaningful interpretation of indentation test results. by Emily Pfeiffer. S.B. 2008-02-27T22:28:18Z 2008-02-27T22:28:18Z 2007 2007 Thesis http://hdl.handle.net/1721.1/40463 191733957 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 42 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Pfeiffer, Emily (Emily R.) The effects of glycosaminoglycan content on the compressive modulus of chondrocyte seeded type II collagen scaffolds |
| title | The effects of glycosaminoglycan content on the compressive modulus of chondrocyte seeded type II collagen scaffolds |
| title_full | The effects of glycosaminoglycan content on the compressive modulus of chondrocyte seeded type II collagen scaffolds |
| title_fullStr | The effects of glycosaminoglycan content on the compressive modulus of chondrocyte seeded type II collagen scaffolds |
| title_full_unstemmed | The effects of glycosaminoglycan content on the compressive modulus of chondrocyte seeded type II collagen scaffolds |
| title_short | The effects of glycosaminoglycan content on the compressive modulus of chondrocyte seeded type II collagen scaffolds |
| title_sort | effects of glycosaminoglycan content on the compressive modulus of chondrocyte seeded type ii collagen scaffolds |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/40463 |
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