System to study colony-colony interactions in embryonic stem cells
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2007.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2008
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| Online Access: | http://hdl.handle.net/1721.1/40880 |
| _version_ | 1811080251871068160 |
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| author | Sampattavanich, Somponnat |
| author2 | Joel Voldman. |
| author_facet | Joel Voldman. Sampattavanich, Somponnat |
| author_sort | Sampattavanich, Somponnat |
| collection | MIT |
| description | Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2007. |
| first_indexed | 2024-09-23T11:28:15Z |
| format | Thesis |
| id | mit-1721.1/40880 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T11:28:15Z |
| publishDate | 2008 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/408802019-04-12T09:40:23Z System to study colony-colony interactions in embryonic stem cells Sampattavanich, Somponnat Joel Voldman. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Electrical Engineering and Computer Science. Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2007. Includes bibliographical references (p. 101-108). Many efforts have been made to characterize the necessary regulatory factors involved in self-renewal of embryonic stem cells (ESCs). Recent studies suggested that different autocrine factors exist in murine ESCs (mESCs) and can influence their self-renewal and proliferation, predominantly in deficiency conditions. These observations were investigated based on a comparison of different groups of mESCs that were plated at varying cell densities. In this study, we developed an experimental platform to study colony-colony interactions in mESCs. We have used stencil cell patterning to precisely localize mESC colonies on the culture substrate. This technique allows the formation of mESC colonies with precise shape and controllable inter-colony distances. We monitored colony proliferation, motility and fusion in response to different initial colony configurations over time using the developed image analysis algorithms and immunohistochemistry techniques. Different cultivating conditions commonly used for mESCs were tested to identify the environment where autocrine signaling is significant. by Somponnat Sampattavanich. S.M. 2008-03-27T18:18:14Z 2008-03-27T18:18:14Z 2007 2007 Thesis http://hdl.handle.net/1721.1/40880 191958735 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 108 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Electrical Engineering and Computer Science. Sampattavanich, Somponnat System to study colony-colony interactions in embryonic stem cells |
| title | System to study colony-colony interactions in embryonic stem cells |
| title_full | System to study colony-colony interactions in embryonic stem cells |
| title_fullStr | System to study colony-colony interactions in embryonic stem cells |
| title_full_unstemmed | System to study colony-colony interactions in embryonic stem cells |
| title_short | System to study colony-colony interactions in embryonic stem cells |
| title_sort | system to study colony colony interactions in embryonic stem cells |
| topic | Electrical Engineering and Computer Science. |
| url | http://hdl.handle.net/1721.1/40880 |
| work_keys_str_mv | AT sampattavanichsomponnat systemtostudycolonycolonyinteractionsinembryonicstemcells |