Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2008.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2009
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| Online Access: | http://hdl.handle.net/1721.1/44853 |
| _version_ | 1826215942422528000 |
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| author | Ueda, Erica (Erica Ann) |
| author2 | Myron Spector. |
| author_facet | Myron Spector. Ueda, Erica (Erica Ann) |
| author_sort | Ueda, Erica (Erica Ann) |
| collection | MIT |
| description | Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2008. |
| first_indexed | 2024-09-23T16:39:54Z |
| format | Thesis |
| id | mit-1721.1/44853 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T16:39:54Z |
| publishDate | 2009 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/448532019-04-10T11:14:09Z Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering Ueda, Erica (Erica Ann) Myron Spector. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Mechanical Engineering. Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2008. Includes bibliographical references (p. 123-125). Rat neural stem cells (NSCs) were cultured in monolayer or in porous collagen scaffolds and exposed to neurogenic or non-neurogenic medium to determine the effects on neural differentiation and neurite growth. Nestin, [beta]III-tubulin, and GFAP expression were determined using immunofluorescent techniques, and the neurite length was measured. NSCs differentiated into neurons, with actively growing neurites, and astrocytes when cultured in differentiation medium (DM) or neurogenic medium (NM). NSCs cultured in monolayer expressed more nestin and III-tubulin and had significantly longer neurite extensions than NSCs cultured in collagen scaffolds. Laminin coated scaffolds promoted the attachment of NSCs to the scaffold struts and resulted in a more even distribution of nestin and [beta]III-tubulin positive cells throughout the scaffold. Overall, NSCs cultured in DM for at least 14 days resulted in the most neuronal differentiation and neurite growth. by Erica Ueda. S.M. 2009-03-16T19:51:20Z 2009-03-16T19:51:20Z 2008 2008 Thesis http://hdl.handle.net/1721.1/44853 301735192 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 125 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Ueda, Erica (Erica Ann) Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering |
| title | Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering |
| title_full | Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering |
| title_fullStr | Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering |
| title_full_unstemmed | Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering |
| title_short | Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering |
| title_sort | neural stem cell differentiation in collagen scaffolds for retinal tissue engineering |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/44853 |
| work_keys_str_mv | AT uedaericaericaann neuralstemcelldifferentiationincollagenscaffoldsforretinaltissueengineering |