Methodology and applications of high resolution solid-state NMR to structure determination of proteins
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2008.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2009
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| Online Access: | http://hdl.handle.net/1721.1/45640 |
| _version_ | 1826188956871426048 |
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| author | Lewandowski, Józef Romuald |
| author2 | Robert G. Griffin. |
| author_facet | Robert G. Griffin. Lewandowski, Józef Romuald |
| author_sort | Lewandowski, Józef Romuald |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2008. |
| first_indexed | 2024-09-23T08:07:36Z |
| format | Thesis |
| id | mit-1721.1/45640 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T08:07:36Z |
| publishDate | 2009 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/456402019-04-09T16:47:02Z Methodology and applications of high resolution solid-state NMR to structure determination of proteins Lewandowski, Józef Romuald Robert G. Griffin. Massachusetts Institute of Technology. Dept. of Chemistry. Massachusetts Institute of Technology. Dept. of Chemistry. Chemistry. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2008. This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections. Vita. Includes bibliographical references. A number of methodological developments and applications of solid-state NMR for assignment and high resolution structure determination of microcrystalline proteins and amyloid fibrils are presented. Magic angle spinning spectroscopy on uniformly and selectively "C and '5N labeled samples is performed at magnetic fields from 11.7 to 21.1 T and spinning frequencies from 9 to 65 kHz.Dynamic Nuclear Polarization on nanocrystals of amyloidogenic peptide GNNQQNY is presented demonstrating that 'H-'H spin diffusion can efficiently transfer the enhanced polarization across the solute that is not in an intimate contact with the polarizing agent.An improved theoretical treatment of Rotational Resonance Width (R2W) experiments and its application to determination of precise 13C-13C distance is presented. A general theory of second averaging in modulation frame for designing solid-state NMR experiments is introduced and discussed in the context of two methods: Cosine Modulated Rotary Resonance (CMpRR) for performing a broadband double-quantum 13C-13C recoupling without the need for additional 'H decoupling and Cosine Modulated recoupling with Chemical Shift reintroduction (COMICS) that provides a general frequency selective method for measuring precise 13C-13C distances in uniformly labeled solids. Cosine Modulated Adiabatic Recoupling (CMAR) - an adiabatic extension of the CMpRR, that is particularly robust with respect to rf inhomogeneity, is also introduced. A number of applications CMpRR at 21.1 T to proteins with varying degrees of macroscopic order are presented. A second order Third Spin Assisted Recoupling (TSAR) mechanism is introduced and discussed in detail. The heteronuclear TSAR - Proton Assisted Insensitive Nuclei Cross-Polarization (PAIN-CP) and homonuclear Proton Assisted Recoupling (PAR) yield long distance 13C_1-N, 3C-_13C and 15N- 5N restraints in uniformly labeled systems with spinning frequencies up to 65 kHz that are used for protein structure calculation. Structure, dynamics and polymorphism of amyloidogenic peptide GNNQQNY from the yeast protein sup35p are investigated. Finally, PAIN-CP and '3C-13C PAR are used for high resolution de novo structure determination of 10.4 kDa Crh protein dimer. by Józef Romuald Lewandowski. Ph.D. 2009-06-25T20:37:19Z 2009-06-25T20:37:19Z 2008 2008 Thesis http://hdl.handle.net/1721.1/45640 370427379 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 439 leaves application/pdf Massachusetts Institute of Technology |
| spellingShingle | Chemistry. Lewandowski, Józef Romuald Methodology and applications of high resolution solid-state NMR to structure determination of proteins |
| title | Methodology and applications of high resolution solid-state NMR to structure determination of proteins |
| title_full | Methodology and applications of high resolution solid-state NMR to structure determination of proteins |
| title_fullStr | Methodology and applications of high resolution solid-state NMR to structure determination of proteins |
| title_full_unstemmed | Methodology and applications of high resolution solid-state NMR to structure determination of proteins |
| title_short | Methodology and applications of high resolution solid-state NMR to structure determination of proteins |
| title_sort | methodology and applications of high resolution solid state nmr to structure determination of proteins |
| topic | Chemistry. |
| url | http://hdl.handle.net/1721.1/45640 |
| work_keys_str_mv | AT lewandowskijozefromuald methodologyandapplicationsofhighresolutionsolidstatenmrtostructuredeterminationofproteins |