Design and characterization of artificial transcriptional terminators
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2007.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2009
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| Online Access: | http://hdl.handle.net/1721.1/45981 |
| _version_ | 1811075584946601984 |
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| author | Huang, Haiyao |
| author2 | Thomas F. Knight, Jr. |
| author_facet | Thomas F. Knight, Jr. Huang, Haiyao |
| author_sort | Huang, Haiyao |
| collection | MIT |
| description | Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2007. |
| first_indexed | 2024-09-23T10:08:49Z |
| format | Thesis |
| id | mit-1721.1/45981 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T10:08:49Z |
| publishDate | 2009 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/459812019-04-12T13:50:46Z Design and characterization of artificial transcriptional terminators Huang, Haiyao Thomas F. Knight, Jr. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Electrical Engineering and Computer Science. Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2007. Includes bibliographical references (p. 103-104). Design and characterization of artificial transcriptional terminators. Ten new terminators were designed based on previous research of terminator structure and termination efficiency. The terminators were built by PCR extension, ligated into a BioBrick plasmid backbone, and transformed into TOP10 cells. Characterization devices were built to test the terminators. Input and output of the terminator were measured by expression of RFP and GFP. Charaterization devices were then placed into the E. coli strain CW2553/pJAT18, which hijacks the arabinose transport system to provide controlled input to the terminator. Of the ten terminators designed and tested, BBa_B1002, BBa_B1004, BBa_B1006 and BBa_B1010 proved to be strong terminators with termination efficiencies above 90%. These terminators may be obtained from the Registry of Standarized Parts at parts.mit.edu. by Haiyao Huang. M.Eng. 2009-06-30T16:54:37Z 2009-06-30T16:54:37Z 2007 2007 Thesis http://hdl.handle.net/1721.1/45981 334966967 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 104 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Electrical Engineering and Computer Science. Huang, Haiyao Design and characterization of artificial transcriptional terminators |
| title | Design and characterization of artificial transcriptional terminators |
| title_full | Design and characterization of artificial transcriptional terminators |
| title_fullStr | Design and characterization of artificial transcriptional terminators |
| title_full_unstemmed | Design and characterization of artificial transcriptional terminators |
| title_short | Design and characterization of artificial transcriptional terminators |
| title_sort | design and characterization of artificial transcriptional terminators |
| topic | Electrical Engineering and Computer Science. |
| url | http://hdl.handle.net/1721.1/45981 |
| work_keys_str_mv | AT huanghaiyao designandcharacterizationofartificialtranscriptionalterminators |