| Summary: | The physical assembly of standard parts is currently a non-standard process, which can either
come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers,
or overlap assembly PCR. Furthermore, current Biobrick construction standards [RFC 10,11,12
etc.] rely heavily on restriction enzyme based methods, which can be sequence, cost and time
restrictive. This is especially true of large DNA assembly, where 100% control of over DNA
sequence may be mandatory, such as in whole genome assembly. We propose a novel standard,
The Building Block Method, for both the construction of standard parts and their assembly
This Building Block standard can be either A) an interchangeable or B) a non-interchangeable
format, based on the desired use of the part.
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