BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction
The physical assembly of standard parts is currently a non-standard process, which can either come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers, or overlap assembly PCR. Furthermore, current Biobrick construction standards [RFC 10,11,12 etc.] rely heav...
| Main Authors: | , |
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| Format: | Technical Report |
| Language: | en_US |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://hdl.handle.net/1721.1/49417 |
| _version_ | 1826196234075897856 |
|---|---|
| author | DiCarlo, James Boeke, Jef |
| author_facet | DiCarlo, James Boeke, Jef |
| author_sort | DiCarlo, James |
| collection | MIT |
| description | The physical assembly of standard parts is currently a non-standard process, which can either
come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers,
or overlap assembly PCR. Furthermore, current Biobrick construction standards [RFC 10,11,12
etc.] rely heavily on restriction enzyme based methods, which can be sequence, cost and time
restrictive. This is especially true of large DNA assembly, where 100% control of over DNA
sequence may be mandatory, such as in whole genome assembly. We propose a novel standard,
The Building Block Method, for both the construction of standard parts and their assembly
This Building Block standard can be either A) an interchangeable or B) a non-interchangeable
format, based on the desired use of the part. |
| first_indexed | 2024-09-23T10:23:38Z |
| format | Technical Report |
| id | mit-1721.1/49417 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:23:38Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | mit-1721.1/494172019-04-12T23:25:14Z BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction DiCarlo, James Boeke, Jef technical standards request for comment biobricks synthetic biology The physical assembly of standard parts is currently a non-standard process, which can either come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers, or overlap assembly PCR. Furthermore, current Biobrick construction standards [RFC 10,11,12 etc.] rely heavily on restriction enzyme based methods, which can be sequence, cost and time restrictive. This is especially true of large DNA assembly, where 100% control of over DNA sequence may be mandatory, such as in whole genome assembly. We propose a novel standard, The Building Block Method, for both the construction of standard parts and their assembly This Building Block standard can be either A) an interchangeable or B) a non-interchangeable format, based on the desired use of the part. 2009-10-07T21:51:51Z 2009-10-07T21:51:51Z 2009-10-07T21:51:51Z Technical Report http://hdl.handle.net/1721.1/49417 en_US BBF RFC;38 application/pdf |
| spellingShingle | technical standards request for comment biobricks synthetic biology DiCarlo, James Boeke, Jef BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction |
| title | BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction |
| title_full | BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction |
| title_fullStr | BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction |
| title_full_unstemmed | BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction |
| title_short | BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction |
| title_sort | bbf rfc 38 rfc 38 building blocks standard large dna genome construction |
| topic | technical standards request for comment biobricks synthetic biology |
| url | http://hdl.handle.net/1721.1/49417 |
| work_keys_str_mv | AT dicarlojames bbfrfc38rfc38buildingblocksstandardlargednagenomeconstruction AT boekejef bbfrfc38rfc38buildingblocksstandardlargednagenomeconstruction |