Closing gaps in the human genome using sequencing by synthesis
The most recent release of the finished human genome contains 260 euchromatic gaps (excluding chromosome Y). Recent work has helped explain a large number of these unresolved regions as 'structural' in nature. Another class of gaps is likely to be refractory to clone-based approaches, and...
| Main Authors: | , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
BioMed Central Ltd.
2009
|
| Online Access: | http://hdl.handle.net/1721.1/49494 |
| _version_ | 1826210304043778048 |
|---|---|
| author | Arachchi, Harindra M. Green, Lisa M. Zody, Michael C. Lennon, Niall Gnerre, Sante Berlin, Aaron M. Nusbaum, Chad Garber, Manuel |
| author2 | Broad Institute of MIT and Harvard |
| author_facet | Broad Institute of MIT and Harvard Arachchi, Harindra M. Green, Lisa M. Zody, Michael C. Lennon, Niall Gnerre, Sante Berlin, Aaron M. Nusbaum, Chad Garber, Manuel |
| author_sort | Arachchi, Harindra M. |
| collection | MIT |
| description | The most recent release of the finished human genome contains 260 euchromatic gaps (excluding chromosome Y). Recent work has helped explain a large number of these unresolved regions as 'structural' in nature. Another class of gaps is likely to be refractory to clone-based approaches, and cannot be approached in ways previously described. We present an approach for closing these gaps using 454 sequencing. As a proof of principle, we closed all three remaining non-structural gaps in chromosome 15. |
| first_indexed | 2024-09-23T14:47:41Z |
| format | Article |
| id | mit-1721.1/49494 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:47:41Z |
| publishDate | 2009 |
| publisher | BioMed Central Ltd. |
| record_format | dspace |
| spelling | mit-1721.1/494942022-09-29T10:36:45Z Closing gaps in the human genome using sequencing by synthesis Arachchi, Harindra M. Green, Lisa M. Zody, Michael C. Lennon, Niall Gnerre, Sante Berlin, Aaron M. Nusbaum, Chad Garber, Manuel Broad Institute of MIT and Harvard Gnerre, Sante Berlin, Aaron M. Gnerre, Sante Green, Lisa M. Nusbaum, Chad Lennon, Niall Arachchi, Harindra M. Zody, Michael C. Garber, Manuel The most recent release of the finished human genome contains 260 euchromatic gaps (excluding chromosome Y). Recent work has helped explain a large number of these unresolved regions as 'structural' in nature. Another class of gaps is likely to be refractory to clone-based approaches, and cannot be approached in ways previously described. We present an approach for closing these gaps using 454 sequencing. As a proof of principle, we closed all three remaining non-structural gaps in chromosome 15. 2009-10-19T13:46:52Z 2009-10-19T13:46:52Z 2009-06 2009-04 Article http://purl.org/eprint/type/JournalArticle 1465-6906 http://hdl.handle.net/1721.1/49494 M. Garber, M. Zody, H. Arachchi, A. Berlin, S. Gnerre, L. Green, N. Lennon, and C. Nusbaum, “Closing gaps in the human genome using sequencing by synthesis,” Genome Biology, vol. 10, 2009, p. R60. 19490611 en_US http://dx.doi.org/10.1186/gb-2009-10-6-r60 Genome Biology Creative Commons Attribution http://creativecommons.org/licenses/by/2.0 application/pdf BioMed Central Ltd. Publisher |
| spellingShingle | Arachchi, Harindra M. Green, Lisa M. Zody, Michael C. Lennon, Niall Gnerre, Sante Berlin, Aaron M. Nusbaum, Chad Garber, Manuel Closing gaps in the human genome using sequencing by synthesis |
| title | Closing gaps in the human genome using sequencing by synthesis |
| title_full | Closing gaps in the human genome using sequencing by synthesis |
| title_fullStr | Closing gaps in the human genome using sequencing by synthesis |
| title_full_unstemmed | Closing gaps in the human genome using sequencing by synthesis |
| title_short | Closing gaps in the human genome using sequencing by synthesis |
| title_sort | closing gaps in the human genome using sequencing by synthesis |
| url | http://hdl.handle.net/1721.1/49494 |
| work_keys_str_mv | AT arachchiharindram closinggapsinthehumangenomeusingsequencingbysynthesis AT greenlisam closinggapsinthehumangenomeusingsequencingbysynthesis AT zodymichaelc closinggapsinthehumangenomeusingsequencingbysynthesis AT lennonniall closinggapsinthehumangenomeusingsequencingbysynthesis AT gnerresante closinggapsinthehumangenomeusingsequencingbysynthesis AT berlinaaronm closinggapsinthehumangenomeusingsequencingbysynthesis AT nusbaumchad closinggapsinthehumangenomeusingsequencingbysynthesis AT garbermanuel closinggapsinthehumangenomeusingsequencingbysynthesis |