RA-PCR, a method for the generation of randomized promoter libraries
The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of transcription factors of interest). The method generates a library of promoters pu...
| Main Authors: | , , , , |
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| Format: | Technical Report |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://hdl.handle.net/1721.1/49502 |
| _version_ | 1811083024952983552 |
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| author | Velten, Lars Haas, Simon Rademacher, Anne Meyer, Hannah Eils, Roland |
| author_facet | Velten, Lars Haas, Simon Rademacher, Anne Meyer, Hannah Eils, Roland |
| author_sort | Velten, Lars |
| collection | MIT |
| description | The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of transcription factors of interest). The method generates a library of promoters putatively active under the desired condition. This RFC also provides instructions on how to screen the libraries generated by this method in order to obtain functional promoters. Description of promoters generated by this method can be found at http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters. |
| first_indexed | 2024-09-23T12:18:35Z |
| format | Technical Report |
| id | mit-1721.1/49502 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:18:35Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | mit-1721.1/495022019-04-10T07:38:59Z RA-PCR, a method for the generation of randomized promoter libraries Velten, Lars Haas, Simon Rademacher, Anne Meyer, Hannah Eils, Roland method The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of transcription factors of interest). The method generates a library of promoters putatively active under the desired condition. This RFC also provides instructions on how to screen the libraries generated by this method in order to obtain functional promoters. Description of promoters generated by this method can be found at http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters. 2009-10-21T01:24:36Z 2009-10-21T01:24:36Z 2009-10-21T01:24:36Z Technical Report http://hdl.handle.net/1721.1/49502 en BBF RFC;42 application/pdf |
| spellingShingle | method Velten, Lars Haas, Simon Rademacher, Anne Meyer, Hannah Eils, Roland RA-PCR, a method for the generation of randomized promoter libraries |
| title | RA-PCR, a method for the generation of randomized promoter libraries |
| title_full | RA-PCR, a method for the generation of randomized promoter libraries |
| title_fullStr | RA-PCR, a method for the generation of randomized promoter libraries |
| title_full_unstemmed | RA-PCR, a method for the generation of randomized promoter libraries |
| title_short | RA-PCR, a method for the generation of randomized promoter libraries |
| title_sort | ra pcr a method for the generation of randomized promoter libraries |
| topic | method |
| url | http://hdl.handle.net/1721.1/49502 |
| work_keys_str_mv | AT veltenlars rapcramethodforthegenerationofrandomizedpromoterlibraries AT haassimon rapcramethodforthegenerationofrandomizedpromoterlibraries AT rademacheranne rapcramethodforthegenerationofrandomizedpromoterlibraries AT meyerhannah rapcramethodforthegenerationofrandomizedpromoterlibraries AT eilsroland rapcramethodforthegenerationofrandomizedpromoterlibraries |