Compartmentalization evaluated to explain discrepancies calculating cartilage fixed charge density
Thesis (M.Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1998.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2010
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| Online Access: | http://hdl.handle.net/1721.1/50516 |
| _version_ | 1811080396364840960 |
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| author | Thomas, Arun Mammen, 1974- |
| author2 | Martha L. Gray. |
| author_facet | Martha L. Gray. Thomas, Arun Mammen, 1974- |
| author_sort | Thomas, Arun Mammen, 1974- |
| collection | MIT |
| description | Thesis (M.Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1998. |
| first_indexed | 2024-09-23T11:30:59Z |
| format | Thesis |
| id | mit-1721.1/50516 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T11:30:59Z |
| publishDate | 2010 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/505162019-04-11T10:33:46Z Compartmentalization evaluated to explain discrepancies calculating cartilage fixed charge density Thomas, Arun Mammen, 1974- Martha L. Gray. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science Electrical Engineering and Computer Science Thesis (M.Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1998. Includes bibliographical references (leaves 83-87). Recent studies on methods of detecting the early onset of arthritic cartilage degradation using NMR-based techniques have shown that such detection is possible. The use of sodium NMR observations, along with an ideal Donnan single compartment model of cartilage, has already been validated as a means of measuring cartilage fixed charge density - a known indicator of cartilage condition. Similar calculations of fixed charge density from proton (in the presence of gadopentetate) NMR observations and the same model were highly correlated with, but 50% below, values derived from sodium NMR. Maroudas had previously shown the water content of cartilage to be divided with a roughly 30:70 ratio between two physiologically distinct regions. The first of these regions, within cartilage collagen fibrils, is electroneutral, with most of the tissue fixed charge, in the form of chondroitin sulfate, being concentrated in the remainder of the tissue, the second region. The existence of two compartments, with different associated fixed charge densities, is shown, by spreadsheet computations and analysis of previously published data, to be a possible reason for the observed 50% discrepancy. High-concentration chondroitin sulfate solutions within dialysis tubing bags were equilibrated in solutions containing sodium and gadopentetate ions. This solution/tubing apparatus mimicked an ideal Donnan single compartment. MR measurements of the amounts of the two ions and calculations of fixed charge density in the solutions based on the measurements yielded the same 50% factor. Since this artificial model did not include any collagen, there were no compartmentalization effects due to structural factors. A similar single-compartment was done using a non-ionic contrast agent to test for steric exclusion based compartmentalization. Although MR measurements revealed a discrepancy in contrast agent distribution, the discrepancy was exactly opposite what should have been observed had steric exclusion been a factor. In summary, it seemed clear that compartmentalization of water (from either cartilage structure or from steric exclusion) was not primarily responsible for the observed 50% discrepancy. Another explanation must be found. by Arun Mammen Thomas. M.Eng. 2010-01-07T20:48:08Z 2010-01-07T20:48:08Z 1998 1998 Thesis http://hdl.handle.net/1721.1/50516 42429642 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 87 leaves application/pdf Massachusetts Institute of Technology |
| spellingShingle | Electrical Engineering and Computer Science Thomas, Arun Mammen, 1974- Compartmentalization evaluated to explain discrepancies calculating cartilage fixed charge density |
| title | Compartmentalization evaluated to explain discrepancies calculating cartilage fixed charge density |
| title_full | Compartmentalization evaluated to explain discrepancies calculating cartilage fixed charge density |
| title_fullStr | Compartmentalization evaluated to explain discrepancies calculating cartilage fixed charge density |
| title_full_unstemmed | Compartmentalization evaluated to explain discrepancies calculating cartilage fixed charge density |
| title_short | Compartmentalization evaluated to explain discrepancies calculating cartilage fixed charge density |
| title_sort | compartmentalization evaluated to explain discrepancies calculating cartilage fixed charge density |
| topic | Electrical Engineering and Computer Science |
| url | http://hdl.handle.net/1721.1/50516 |
| work_keys_str_mv | AT thomasarunmammen1974 compartmentalizationevaluatedtoexplaindiscrepanciescalculatingcartilagefixedchargedensity |