Hook3 interacts with PCM1 to regulate pericentriolar material assembly and the timing of neurogenesis
Centrosome functions are important in many brain developmental processes. Proper functioning of the centrosome relies on assembly of protein components into the pericentriolar material. This dynamic assembly is mediated by the trafficking of pericentriolar satellites, which are comprised of centr...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier
2010
|
| Online Access: | http://hdl.handle.net/1721.1/50868 https://orcid.org/0000-0003-1262-0592 |
| _version_ | 1826205568645201920 |
|---|---|
| author | Ge, Xuecai Frank, Christopher Lee Calderon de Anda, Froylan Tsai, Li-Huei |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Ge, Xuecai Frank, Christopher Lee Calderon de Anda, Froylan Tsai, Li-Huei |
| author_sort | Ge, Xuecai |
| collection | MIT |
| description | Centrosome functions are important in many brain developmental processes. Proper
functioning of the centrosome relies on assembly of protein components into the pericentriolar
material. This dynamic assembly is mediated by the trafficking of pericentriolar satellites, which are
comprised of centrosomal proteins. Here we demonstrate that trafficking of pericentriolar satellites
requires the interaction between Hook3 and Pericentriolar Material 1 (PCM1). Hook3, previously
shown to link the centrosome and the nucleus in C. elegans, is recruited to pericentriolar satellites
through interaction with PCM1, a protein associated with schizophrenia. Knocking down of Hook3 or
PCM1, or disrupting the Hook3-PCM1 interaction in vivo impairs interkinetic nuclear migration, a
featured behavior of embryonic neural progenitors. This in turn leads to overproduction of neurons
and premature depletion of the neural progenitor pool in the developing neocortex. These results
underscore the importance of centrosomal assembly in neurogenesis, and provide potential insights
into the etiology of brain developmental diseases related to centrosome dysfunction. |
| first_indexed | 2024-09-23T13:15:12Z |
| format | Article |
| id | mit-1721.1/50868 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:15:12Z |
| publishDate | 2010 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/508682022-10-01T14:01:00Z Hook3 interacts with PCM1 to regulate pericentriolar material assembly and the timing of neurogenesis Ge, Xuecai Frank, Christopher Lee Calderon de Anda, Froylan Tsai, Li-Huei Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Picower Institute for Learning and Memory Tsai, Li-Huei Ge, Xuecai Calderon de Anda, Froylan Tsai, Li-Huei Centrosome functions are important in many brain developmental processes. Proper functioning of the centrosome relies on assembly of protein components into the pericentriolar material. This dynamic assembly is mediated by the trafficking of pericentriolar satellites, which are comprised of centrosomal proteins. Here we demonstrate that trafficking of pericentriolar satellites requires the interaction between Hook3 and Pericentriolar Material 1 (PCM1). Hook3, previously shown to link the centrosome and the nucleus in C. elegans, is recruited to pericentriolar satellites through interaction with PCM1, a protein associated with schizophrenia. Knocking down of Hook3 or PCM1, or disrupting the Hook3-PCM1 interaction in vivo impairs interkinetic nuclear migration, a featured behavior of embryonic neural progenitors. This in turn leads to overproduction of neurons and premature depletion of the neural progenitor pool in the developing neocortex. These results underscore the importance of centrosomal assembly in neurogenesis, and provide potential insights into the etiology of brain developmental diseases related to centrosome dysfunction. 2010-01-21T17:13:48Z 2010-01-21T17:13:48Z 2010-01 2010 Article http://purl.org/eprint/type/SubmittedJournalArticle 0896-6273 1097-4199 http://hdl.handle.net/1721.1/50868 Ge, Xuecai, et al. "Hook3 interacts with PCM1 to regulate pericentriolar material assembly and the timing of neurogenesis." Neuron, 65:2, 28 January 2010, Pages 191–203. https://orcid.org/0000-0003-1262-0592 en_US http://dx.doi.org/10.1016/j.neuron.2010.01.011 Neuron Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Li-Huei Tsai via Andy Devlin |
| spellingShingle | Ge, Xuecai Frank, Christopher Lee Calderon de Anda, Froylan Tsai, Li-Huei Hook3 interacts with PCM1 to regulate pericentriolar material assembly and the timing of neurogenesis |
| title | Hook3 interacts with PCM1 to regulate pericentriolar material assembly and the timing of neurogenesis |
| title_full | Hook3 interacts with PCM1 to regulate pericentriolar material assembly and the timing of neurogenesis |
| title_fullStr | Hook3 interacts with PCM1 to regulate pericentriolar material assembly and the timing of neurogenesis |
| title_full_unstemmed | Hook3 interacts with PCM1 to regulate pericentriolar material assembly and the timing of neurogenesis |
| title_short | Hook3 interacts with PCM1 to regulate pericentriolar material assembly and the timing of neurogenesis |
| title_sort | hook3 interacts with pcm1 to regulate pericentriolar material assembly and the timing of neurogenesis |
| url | http://hdl.handle.net/1721.1/50868 https://orcid.org/0000-0003-1262-0592 |
| work_keys_str_mv | AT gexuecai hook3interactswithpcm1toregulatepericentriolarmaterialassemblyandthetimingofneurogenesis AT frankchristopherlee hook3interactswithpcm1toregulatepericentriolarmaterialassemblyandthetimingofneurogenesis AT calderondeandafroylan hook3interactswithpcm1toregulatepericentriolarmaterialassemblyandthetimingofneurogenesis AT tsailihuei hook3interactswithpcm1toregulatepericentriolarmaterialassemblyandthetimingofneurogenesis |