Optical diffraction tomography for high resolution live cell imaging
We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. Using a heterodyne Mach-Zehnder interferometer, we record complex field images of light transmitted through a sample with varying directions of il...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Optical Society of America
2010
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| Online Access: | http://hdl.handle.net/1721.1/51357 https://orcid.org/0000-0002-4568-3218 |
| Summary: | We report the experimental implementation of optical diffraction
tomography for quantitative 3D mapping of refractive index in live
biological cells. Using a heterodyne Mach-Zehnder interferometer, we
record complex field images of light transmitted through a sample with
varying directions of illumination. To quantitatively reconstruct the 3D map
of complex refractive index in live cells, we apply optical diffraction
tomography based on the Rytov approximation. In this way, the effect of
diffraction is taken into account in the reconstruction process and
diffraction-free high resolution 3D images are obtained throughout the
entire sample volume. The quantitative refractive index map can potentially
serve as an intrinsic assay to provide the molecular concentrations without
the addition of exogenous agents and also to provide a method for studying
the light scattering properties of single cells. |
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