Optical diffraction tomography for high resolution live cell imaging
We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. Using a heterodyne Mach-Zehnder interferometer, we record complex field images of light transmitted through a sample with varying directions of il...
| Autores principales: | , , , , , |
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| Otros Autores: | |
| Formato: | Artículo |
| Lenguaje: | en_US |
| Publicado: |
Optical Society of America
2010
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| Acceso en línea: | http://hdl.handle.net/1721.1/51357 https://orcid.org/0000-0002-4568-3218 |
| _version_ | 1826192945396580352 |
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| author | Sung, Yongjin Choi, Wonshik Fang-Yen, Chris Badizadegan, Kamran Dasari, Ramachandra Rao Feld, Michael S. |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Sung, Yongjin Choi, Wonshik Fang-Yen, Chris Badizadegan, Kamran Dasari, Ramachandra Rao Feld, Michael S. |
| author_sort | Sung, Yongjin |
| collection | MIT |
| description | We report the experimental implementation of optical diffraction
tomography for quantitative 3D mapping of refractive index in live
biological cells. Using a heterodyne Mach-Zehnder interferometer, we
record complex field images of light transmitted through a sample with
varying directions of illumination. To quantitatively reconstruct the 3D map
of complex refractive index in live cells, we apply optical diffraction
tomography based on the Rytov approximation. In this way, the effect of
diffraction is taken into account in the reconstruction process and
diffraction-free high resolution 3D images are obtained throughout the
entire sample volume. The quantitative refractive index map can potentially
serve as an intrinsic assay to provide the molecular concentrations without
the addition of exogenous agents and also to provide a method for studying
the light scattering properties of single cells. |
| first_indexed | 2024-09-23T09:31:16Z |
| format | Article |
| id | mit-1721.1/51357 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:31:16Z |
| publishDate | 2010 |
| publisher | Optical Society of America |
| record_format | dspace |
| spelling | mit-1721.1/513572022-09-30T14:59:26Z Optical diffraction tomography for high resolution live cell imaging Sung, Yongjin Choi, Wonshik Fang-Yen, Chris Badizadegan, Kamran Dasari, Ramachandra Rao Feld, Michael S. Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Department of Physics Massachusetts Institute of Technology. Spectroscopy Laboratory Sung, Yongjin Sung, Yongjin Choi, Wonshik Fang-Yen, Chris Badizadegan, Kamran Dasari, Ramachandra Rao Feld, Michael S. We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. Using a heterodyne Mach-Zehnder interferometer, we record complex field images of light transmitted through a sample with varying directions of illumination. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells. 2010-02-04T14:17:06Z 2010-02-04T14:17:06Z 2009-01 2008-11 Article http://purl.org/eprint/type/SubmittedJournalArticle 1094-4087 http://hdl.handle.net/1721.1/51357 Yongjin Sung, Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld, "Optical diffraction tomography for high resolution live cell imaging," Opt. Express 17, 266-277 (2009) http://www.opticsinfobase.org/abstract.cfm?URI=oe-17-1-266 https://orcid.org/0000-0002-4568-3218 en_US http://dx.doi.org/10.1364/OE.17.000266 Optics Express Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Optical Society of America Yongjin Sung |
| spellingShingle | Sung, Yongjin Choi, Wonshik Fang-Yen, Chris Badizadegan, Kamran Dasari, Ramachandra Rao Feld, Michael S. Optical diffraction tomography for high resolution live cell imaging |
| title | Optical diffraction tomography for high resolution live cell imaging |
| title_full | Optical diffraction tomography for high resolution live cell imaging |
| title_fullStr | Optical diffraction tomography for high resolution live cell imaging |
| title_full_unstemmed | Optical diffraction tomography for high resolution live cell imaging |
| title_short | Optical diffraction tomography for high resolution live cell imaging |
| title_sort | optical diffraction tomography for high resolution live cell imaging |
| url | http://hdl.handle.net/1721.1/51357 https://orcid.org/0000-0002-4568-3218 |
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