Physico-chemical characterization of the native and mutant protein cochlin, and its role in adult-onset hearing and balance loss
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Physics, 2009.
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
| Published: |
Massachusetts Institute of Technology
2010
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/1721.1/51602 |
| _version_ | 1826193364236632064 |
|---|---|
| author | Grannan, Benjamin L. (Benjamin Lucas) |
| author2 | George B. Benedek. |
| author_facet | George B. Benedek. Grannan, Benjamin L. (Benjamin Lucas) |
| author_sort | Grannan, Benjamin L. (Benjamin Lucas) |
| collection | MIT |
| description | Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Physics, 2009. |
| first_indexed | 2024-09-23T09:37:54Z |
| format | Thesis |
| id | mit-1721.1/51602 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T09:37:54Z |
| publishDate | 2010 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/516022019-04-12T09:20:20Z Physico-chemical characterization of the native and mutant protein cochlin, and its role in adult-onset hearing and balance loss Grannan, Benjamin L. (Benjamin Lucas) George B. Benedek. Massachusetts Institute of Technology. Dept. of Physics. Massachusetts Institute of Technology. Dept. of Physics. Physics. Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Physics, 2009. Includes bibliographical references (p. 39-40). This thesis investigates the role of the protein cochlin and its isoforms in DFNA9 autosomal dominant late onset senorineural loss and vestibular disorder by quantifying the concentration of cochlin in the inner fluid called perilymph. Through the use of affinity chromatography, high performance liquid chromatography, the Bradford assay, western blot analysis, and proteomics analysis by mass spectroscopy, I acquired data which suggests that the concentration of cochlin in the perilymph of bovine calf ears is at most on the order of 10- mg/ml. I also determined the total protein concentration of native bovine perilymph to be 2.1+0.2 mg/ml. Additionally, I discuss the theory of quasielastic light scattering along with its relevance to understanding the role of cochlin in DFNA9. by Benjamin L. Grannan. S.B. 2010-02-09T16:50:01Z 2010-02-09T16:50:01Z 2009 2009 Thesis http://hdl.handle.net/1721.1/51602 495719792 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 40 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Physics. Grannan, Benjamin L. (Benjamin Lucas) Physico-chemical characterization of the native and mutant protein cochlin, and its role in adult-onset hearing and balance loss |
| title | Physico-chemical characterization of the native and mutant protein cochlin, and its role in adult-onset hearing and balance loss |
| title_full | Physico-chemical characterization of the native and mutant protein cochlin, and its role in adult-onset hearing and balance loss |
| title_fullStr | Physico-chemical characterization of the native and mutant protein cochlin, and its role in adult-onset hearing and balance loss |
| title_full_unstemmed | Physico-chemical characterization of the native and mutant protein cochlin, and its role in adult-onset hearing and balance loss |
| title_short | Physico-chemical characterization of the native and mutant protein cochlin, and its role in adult-onset hearing and balance loss |
| title_sort | physico chemical characterization of the native and mutant protein cochlin and its role in adult onset hearing and balance loss |
| topic | Physics. |
| url | http://hdl.handle.net/1721.1/51602 |
| work_keys_str_mv | AT grannanbenjaminlbenjaminlucas physicochemicalcharacterizationofthenativeandmutantproteincochlinanditsroleinadultonsethearingandbalanceloss |