In silico estimates of cell electroporation by electrical incapacitation waveforms
We use a system model of a cell and approximate magnitudes of electrical incapacitation (EI) device waveforms to estimate conditions that lead to responses with or without electroporation (EP) of cell membranes near electrodes. Single pulse waveforms of Taser X26 and Aegis MK63 devices were measured...
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| Format: | Article |
| Language: | en_US |
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Institute of Electrical and Electronics Engineers
2010
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| Online Access: | http://hdl.handle.net/1721.1/52601 https://orcid.org/0000-0002-9016-5962 |
| _version_ | 1826190186508189696 |
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| author | Weaver, James C. Burns, Stephen K. Smith, Kyle Christopher Esser, Axel Thomas Gowrishankar, Thiruvallur R. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Weaver, James C. Burns, Stephen K. Smith, Kyle Christopher Esser, Axel Thomas Gowrishankar, Thiruvallur R. |
| author_sort | Weaver, James C. |
| collection | MIT |
| description | We use a system model of a cell and approximate magnitudes of electrical incapacitation (EI) device waveforms to estimate conditions that lead to responses with or without electroporation (EP) of cell membranes near electrodes. Single pulse waveforms of Taser X26 and Aegis MK63 devices were measured using a resistive load. For the present estimates the digitized waveforms were scaled in magnitude according to the inverse square radial distance from two tissue-penetrating electrodes, approximated as hemispheres. The corresponding tissue level electric fields were then used as inputs to the cell system model. A dynamic pore model for membrane electroporation (EP) was assigned to many different sites on the cell plasma membrane (PM). EI devices generate sufficiently large transmembrane voltage, U[subscript m](t), such that pores were created, evolving into a heterogeneous and time-dependent pore population. These approximate responses suggest that both waveforms can cause PM EP. Peripheral nerve damage by EP is a candidate side effect. More extensive EP is expected from the Taser X26 than the Aegis MK63, mainly due to the approximately eight-fold difference in the peak magnitudes. In silico examination of EI waveforms by multiscale modeling is warranted, and can involve whole body, tissue and cell level models that now exist and are rapidly being improved. |
| first_indexed | 2024-09-23T08:36:24Z |
| format | Article |
| id | mit-1721.1/52601 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:36:24Z |
| publishDate | 2010 |
| publisher | Institute of Electrical and Electronics Engineers |
| record_format | dspace |
| spelling | mit-1721.1/526012022-09-30T09:55:36Z In silico estimates of cell electroporation by electrical incapacitation waveforms Weaver, James C. Burns, Stephen K. Smith, Kyle Christopher Esser, Axel Thomas Gowrishankar, Thiruvallur R. Harvard University--MIT Division of Health Sciences and Technology Gowrishankar, Thiruvallur R. Weaver, James C. Burns, Stephen K. Smith, Kyle Christopher Esser, Axel Thomas Gowrishankar, Thiruvallur R. We use a system model of a cell and approximate magnitudes of electrical incapacitation (EI) device waveforms to estimate conditions that lead to responses with or without electroporation (EP) of cell membranes near electrodes. Single pulse waveforms of Taser X26 and Aegis MK63 devices were measured using a resistive load. For the present estimates the digitized waveforms were scaled in magnitude according to the inverse square radial distance from two tissue-penetrating electrodes, approximated as hemispheres. The corresponding tissue level electric fields were then used as inputs to the cell system model. A dynamic pore model for membrane electroporation (EP) was assigned to many different sites on the cell plasma membrane (PM). EI devices generate sufficiently large transmembrane voltage, U[subscript m](t), such that pores were created, evolving into a heterogeneous and time-dependent pore population. These approximate responses suggest that both waveforms can cause PM EP. Peripheral nerve damage by EP is a candidate side effect. More extensive EP is expected from the Taser X26 than the Aegis MK63, mainly due to the approximately eight-fold difference in the peak magnitudes. In silico examination of EI waveforms by multiscale modeling is warranted, and can involve whole body, tissue and cell level models that now exist and are rapidly being improved. National Institutes of Health (Grant RO1-GM63857) Aegis Industries, Inc. 2010-03-15T20:24:02Z 2010-03-15T20:24:02Z 2009-11 Article http://purl.org/eprint/type/ConferencePaper 978-1-4244-3296-7 1557-170X http://hdl.handle.net/1721.1/52601 Gowrishankar, T.R. et al. “In silico estimates of cell electroporation by electrical incapacitation waveforms.” Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE. 2009. 6505-6508. © 2009 IEEE https://orcid.org/0000-0002-9016-5962 en_US http://dx.doi.org/10.1109/IEMBS.2009.5333138 2009 Engineering in Medicine and Biology Society Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Institute of Electrical and Electronics Engineers IEEE |
| spellingShingle | Weaver, James C. Burns, Stephen K. Smith, Kyle Christopher Esser, Axel Thomas Gowrishankar, Thiruvallur R. In silico estimates of cell electroporation by electrical incapacitation waveforms |
| title | In silico estimates of cell electroporation by electrical incapacitation waveforms |
| title_full | In silico estimates of cell electroporation by electrical incapacitation waveforms |
| title_fullStr | In silico estimates of cell electroporation by electrical incapacitation waveforms |
| title_full_unstemmed | In silico estimates of cell electroporation by electrical incapacitation waveforms |
| title_short | In silico estimates of cell electroporation by electrical incapacitation waveforms |
| title_sort | in silico estimates of cell electroporation by electrical incapacitation waveforms |
| url | http://hdl.handle.net/1721.1/52601 https://orcid.org/0000-0002-9016-5962 |
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