Ultraviolet refractometry using field-based light scattering spectroscopy
Accurate refractive index measurement in the deep ultraviolet (UV) range is important for the separate quantification of biomolecules such as proteins and DNA in biology. This task is demanding and has not been fully exploited so far. Here we report a new method of measuring refractive index using f...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Optical Society of America
2010
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| Online Access: | http://hdl.handle.net/1721.1/52643 |
| _version_ | 1811080574416191488 |
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| author | Fu, Dan Choi, Wonshik Sung, Yongjin Oh, Seungeun Yaqoob, Zahid Park, YongKeun Dasari, Ramachandra Rao Feld, Michael S. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Fu, Dan Choi, Wonshik Sung, Yongjin Oh, Seungeun Yaqoob, Zahid Park, YongKeun Dasari, Ramachandra Rao Feld, Michael S. |
| author_sort | Fu, Dan |
| collection | MIT |
| description | Accurate refractive index measurement in the deep ultraviolet (UV) range is important for the separate quantification of biomolecules such as proteins and DNA in biology. This task is demanding and has not been fully exploited so far. Here we report a new method of measuring refractive index using field-based light scattering spectroscopy, which is applicable to any wavelength range and suitable for both solutions and homogenous objects with well-defined shape such as microspheres. The angular scattering distribution of single microspheres immersed in homogeneous media is measured over the wavelength range 260 to 315 nm using quantitative phase microscopy. By least square fitting the observed scattering distribution with Mie scattering theory, the refractive index of either the sphere or the immersion medium can be determined provided that one is known a priori. Using this method, we have measured the refractive index dispersion of SiO2 spheres and bovine serum albumin (BSA) solutions in the deep UV region. Specific refractive index increments of BSA are also extracted. Typical accuracy of the present refractive index technique is ≤0.003. The precision of refractive index measurements is ≤0.002 and that of specific refractive index increment determination is ≤0.01 mL/g. |
| first_indexed | 2024-09-23T11:33:40Z |
| format | Article |
| id | mit-1721.1/52643 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:33:40Z |
| publishDate | 2010 |
| publisher | Optical Society of America |
| record_format | dspace |
| spelling | mit-1721.1/526432022-09-27T20:21:17Z Ultraviolet refractometry using field-based light scattering spectroscopy Fu, Dan Choi, Wonshik Sung, Yongjin Oh, Seungeun Yaqoob, Zahid Park, YongKeun Dasari, Ramachandra Rao Feld, Michael S. Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Department of Physics Massachusetts Institute of Technology. Spectroscopy Laboratory Feld, Michael S. Dasari, Ramachandra Rao Feld, Michael S. Park, Yongkeun Yaqoob, Zahid Oh, Seungeun Sung, Yongjin Choi, Wonshik Fu, Dan Accurate refractive index measurement in the deep ultraviolet (UV) range is important for the separate quantification of biomolecules such as proteins and DNA in biology. This task is demanding and has not been fully exploited so far. Here we report a new method of measuring refractive index using field-based light scattering spectroscopy, which is applicable to any wavelength range and suitable for both solutions and homogenous objects with well-defined shape such as microspheres. The angular scattering distribution of single microspheres immersed in homogeneous media is measured over the wavelength range 260 to 315 nm using quantitative phase microscopy. By least square fitting the observed scattering distribution with Mie scattering theory, the refractive index of either the sphere or the immersion medium can be determined provided that one is known a priori. Using this method, we have measured the refractive index dispersion of SiO2 spheres and bovine serum albumin (BSA) solutions in the deep UV region. Specific refractive index increments of BSA are also extracted. Typical accuracy of the present refractive index technique is ≤0.003. The precision of refractive index measurements is ≤0.002 and that of specific refractive index increment determination is ≤0.01 mL/g. Hamamatsu Corporation National Science Foundation (DBI-0754339) National Center for Research Resources of the National Institutes of Health (P41-RR02594-18) 2010-03-17T13:15:52Z 2010-03-17T13:15:52Z 2009-10 2009-09 Article http://purl.org/eprint/type/SubmittedJournalArticle 1094-4087 http://hdl.handle.net/1721.1/52643 Dan Fu, Wonshik Choi, Yongjin Sung, Seungeun Oh, Zahid Yaqoob, Yongkeun Park, Ramachandra R. Dasari, and Michael S. Feld, "Ultraviolet refractometry using field-based light scattering spectroscopy," Opt. Express 17, 18878-18886 (2009) http://www.opticsinfobase.org/abstract.cfm?URI=oe-17-21-18878 en_US http://dx.doi.org/10.1364/OE.17.018878 Optics Express Attribution-Noncommercial-Share Alike 3.0 Unported http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Optical Society of America Gabriel Holbrow |
| spellingShingle | Fu, Dan Choi, Wonshik Sung, Yongjin Oh, Seungeun Yaqoob, Zahid Park, YongKeun Dasari, Ramachandra Rao Feld, Michael S. Ultraviolet refractometry using field-based light scattering spectroscopy |
| title | Ultraviolet refractometry using field-based light scattering spectroscopy |
| title_full | Ultraviolet refractometry using field-based light scattering spectroscopy |
| title_fullStr | Ultraviolet refractometry using field-based light scattering spectroscopy |
| title_full_unstemmed | Ultraviolet refractometry using field-based light scattering spectroscopy |
| title_short | Ultraviolet refractometry using field-based light scattering spectroscopy |
| title_sort | ultraviolet refractometry using field based light scattering spectroscopy |
| url | http://hdl.handle.net/1721.1/52643 |
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