The rational design of nitric oxide selectivity in single-walled carbon nanotube near infrared fluorescence sensors for biological detection
A major challenge in the synthesis of nanotube or nanowire sensors is imparting selective analyte binding through means other than covalent linkages which compromise electronic and optical properties. We synthesize a 3,4-diaminophenyl-functionalized dextran (DAP-dex) wrapping for single-walled carbo...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2010
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| Online Access: | http://hdl.handle.net/1721.1/52675 https://orcid.org/0000-0003-0771-9889 https://orcid.org/0000-0003-2944-808X |
| _version_ | 1826203545571950592 |
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| author | Kim, Jong-Ho Heller, Daniel A. Jin, Hong Barone, Paul W. Song, Changsik Zhang, Jingqing Trudel, Laura J. Wogan, Gerald N. Tannenbaum, Steven Robert Strano, Michael S. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Kim, Jong-Ho Heller, Daniel A. Jin, Hong Barone, Paul W. Song, Changsik Zhang, Jingqing Trudel, Laura J. Wogan, Gerald N. Tannenbaum, Steven Robert Strano, Michael S. |
| author_sort | Kim, Jong-Ho |
| collection | MIT |
| description | A major challenge in the synthesis of nanotube or nanowire sensors is imparting selective analyte binding through means other than covalent linkages which compromise electronic and optical properties. We synthesize a 3,4-diaminophenyl-functionalized dextran (DAP-dex) wrapping for single-walled carbon nanotubes (SWNT) that imparts rapid and selective fluorescence detection of NO, a messenger for biological signaling. The near infrared (nIR) fluorescence of SWNT[ subscript DAP-dex] is immediately and directly bleached by NO, but not by other reactive nitrogen and oxygen species. This bleaching is reversible and shown to be caused by electron transfer from the top of the valence band of SWNT to the lowest unoccupied molecular orbital (LUMO) of NO. The resulting optical sensor is capable of real-time and spatially resolved detection of NO produced by stimulating NO synthase (iNOS) in macrophage cells. We also demonstrate the potential of the optical sensor for in-vivo detection of NO in a mouse model. |
| first_indexed | 2024-09-23T12:39:03Z |
| format | Article |
| id | mit-1721.1/52675 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:39:03Z |
| publishDate | 2010 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/526752022-09-28T09:12:45Z The rational design of nitric oxide selectivity in single-walled carbon nanotube near infrared fluorescence sensors for biological detection Kim, Jong-Ho Heller, Daniel A. Jin, Hong Barone, Paul W. Song, Changsik Zhang, Jingqing Trudel, Laura J. Wogan, Gerald N. Tannenbaum, Steven Robert Strano, Michael S. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Chemistry Strano, Michael S. Kim, Jong-Ho Heller, Daniel A. Jin, Hong Barone, Paul W. Song, Changsik Zhang, Jingqing Trudel, Laura J. Wogan, Gerald N. Tannenbaum, Steven Robert Strano, Michael S. A major challenge in the synthesis of nanotube or nanowire sensors is imparting selective analyte binding through means other than covalent linkages which compromise electronic and optical properties. We synthesize a 3,4-diaminophenyl-functionalized dextran (DAP-dex) wrapping for single-walled carbon nanotubes (SWNT) that imparts rapid and selective fluorescence detection of NO, a messenger for biological signaling. The near infrared (nIR) fluorescence of SWNT[ subscript DAP-dex] is immediately and directly bleached by NO, but not by other reactive nitrogen and oxygen species. This bleaching is reversible and shown to be caused by electron transfer from the top of the valence band of SWNT to the lowest unoccupied molecular orbital (LUMO) of NO. The resulting optical sensor is capable of real-time and spatially resolved detection of NO produced by stimulating NO synthase (iNOS) in macrophage cells. We also demonstrate the potential of the optical sensor for in-vivo detection of NO in a mouse model. 2010-03-17T17:50:51Z 2010-03-17T17:50:51Z 2009-08 2009-05 Article http://purl.org/eprint/type/SubmittedJournalArticle 1755-4349 http://hdl.handle.net/1721.1/52675 Kim, Jong-Ho et al. “The rational design of nitric oxide selectivity in single-walled carbon nanotube near-infrared fluorescence sensors for biological detection.” Nat Chem 1.6 (2009): 473-481. https://orcid.org/0000-0003-0771-9889 https://orcid.org/0000-0003-2944-808X en_US http://dx.doi.org/10.1038/nchem.332 Nature Chemistry Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group Michael Strano |
| spellingShingle | Kim, Jong-Ho Heller, Daniel A. Jin, Hong Barone, Paul W. Song, Changsik Zhang, Jingqing Trudel, Laura J. Wogan, Gerald N. Tannenbaum, Steven Robert Strano, Michael S. The rational design of nitric oxide selectivity in single-walled carbon nanotube near infrared fluorescence sensors for biological detection |
| title | The rational design of nitric oxide selectivity in single-walled carbon nanotube near infrared fluorescence sensors for biological detection |
| title_full | The rational design of nitric oxide selectivity in single-walled carbon nanotube near infrared fluorescence sensors for biological detection |
| title_fullStr | The rational design of nitric oxide selectivity in single-walled carbon nanotube near infrared fluorescence sensors for biological detection |
| title_full_unstemmed | The rational design of nitric oxide selectivity in single-walled carbon nanotube near infrared fluorescence sensors for biological detection |
| title_short | The rational design of nitric oxide selectivity in single-walled carbon nanotube near infrared fluorescence sensors for biological detection |
| title_sort | rational design of nitric oxide selectivity in single walled carbon nanotube near infrared fluorescence sensors for biological detection |
| url | http://hdl.handle.net/1721.1/52675 https://orcid.org/0000-0003-0771-9889 https://orcid.org/0000-0003-2944-808X |
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