Microfluidic in vivo screen identifies compounds enhancing neuronal
Compound screening is a powerful tool to identify new therapeutic targets, drug leads, and elucidate the fundamental mechanisms of biological processes. We report here the results of the first in vivo small-molecule screens for compounds enhancing neuronal regeneration. These screens are enabled by...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Institute of Electrical and Electronics Engineers
2010
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| Online Access: | http://hdl.handle.net/1721.1/53513 https://orcid.org/0000-0002-4612-1962 |
| _version_ | 1811073539326869504 |
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| author | Haggarty, Stephen J. Norton, Stephanie Samara, Chrysanthi Gilleland, Cody Lee Rohde, Christopher Benjamin Yanik, Mehmet Fatih |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Haggarty, Stephen J. Norton, Stephanie Samara, Chrysanthi Gilleland, Cody Lee Rohde, Christopher Benjamin Yanik, Mehmet Fatih |
| author_sort | Haggarty, Stephen J. |
| collection | MIT |
| description | Compound screening is a powerful tool to identify new therapeutic targets, drug leads, and elucidate the fundamental mechanisms of biological processes. We report here the results of the first in vivo small-molecule screens for compounds enhancing neuronal regeneration. These screens are enabled by the microfluidic devices we have developed for C. elegans. The devices enable rapid and repeatable animal immobilization which allows high-throughput and precise surgery. Following surgery, animals are exposed to the contents of a small-molecule library and assayed for neuronal regeneration. Using this screening method we have identified several compounds that enhance neural regeneration in vivo. |
| first_indexed | 2024-09-23T09:34:34Z |
| format | Article |
| id | mit-1721.1/53513 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:34:34Z |
| publishDate | 2010 |
| publisher | Institute of Electrical and Electronics Engineers |
| record_format | dspace |
| spelling | mit-1721.1/535132022-09-30T15:23:42Z Microfluidic in vivo screen identifies compounds enhancing neuronal Microfluidic in vivo screen identifies compounds enhancing neuronal regeneration Haggarty, Stephen J. Norton, Stephanie Samara, Chrysanthi Gilleland, Cody Lee Rohde, Christopher Benjamin Yanik, Mehmet Fatih Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Yanik, Mehmet Fatih Samara, Chrysanthi Gilleland, Cody Lee Rohde, Christopher Benjamin Yanik, Mehmet Fatih Compound screening is a powerful tool to identify new therapeutic targets, drug leads, and elucidate the fundamental mechanisms of biological processes. We report here the results of the first in vivo small-molecule screens for compounds enhancing neuronal regeneration. These screens are enabled by the microfluidic devices we have developed for C. elegans. The devices enable rapid and repeatable animal immobilization which allows high-throughput and precise surgery. Following surgery, animals are exposed to the contents of a small-molecule library and assayed for neuronal regeneration. Using this screening method we have identified several compounds that enhance neural regeneration in vivo. NIH Director's New Innovator Award Program (1-DP2-OD002989-01) 2010-04-02T19:50:14Z 2010-04-02T19:50:14Z 2009-11 2009-09 Article http://purl.org/eprint/type/ConferencePaper 978-1-4244-3296-7 1557-170X INSPEC Accession Number: 10984060 http://hdl.handle.net/1721.1/53513 Rohde, C.B. et al. “Microfluidic in vivo screen identifies compounds enhancing neuronal regeneration.” Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE. 2009. 5950-5952. © 2009 Institute of Electrical and Electronics Engineers https://orcid.org/0000-0002-4612-1962 en_US http://dx.doi.org/10.1109/IEMBS.2009.5334771 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2009. EMBC 2009. Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Institute of Electrical and Electronics Engineers IEEE |
| spellingShingle | Haggarty, Stephen J. Norton, Stephanie Samara, Chrysanthi Gilleland, Cody Lee Rohde, Christopher Benjamin Yanik, Mehmet Fatih Microfluidic in vivo screen identifies compounds enhancing neuronal |
| title | Microfluidic in vivo screen identifies compounds enhancing neuronal |
| title_full | Microfluidic in vivo screen identifies compounds enhancing neuronal |
| title_fullStr | Microfluidic in vivo screen identifies compounds enhancing neuronal |
| title_full_unstemmed | Microfluidic in vivo screen identifies compounds enhancing neuronal |
| title_short | Microfluidic in vivo screen identifies compounds enhancing neuronal |
| title_sort | microfluidic in vivo screen identifies compounds enhancing neuronal |
| url | http://hdl.handle.net/1721.1/53513 https://orcid.org/0000-0002-4612-1962 |
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