Speckle-field digital holographic microscopy
The use of coherent light in conventional holographic phase microscopy (HPM) poses three major drawbacks: poor spatial resolution, weak depth sectioning, and fixed pattern noise due to unwanted diffraction. Here, we report a technique which can overcome these drawbacks, but maintains the advanta...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Optical Society of America
2010
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| Online Access: | http://hdl.handle.net/1721.1/54778 |
| _version_ | 1826206756605263872 |
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| author | Badizadegan, Kamran Dasari, Ramachandra Rao Yaqoob, Zahid Choi, Wonshik Feld, Michael S. Park, YongKeun |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Badizadegan, Kamran Dasari, Ramachandra Rao Yaqoob, Zahid Choi, Wonshik Feld, Michael S. Park, YongKeun |
| author_sort | Badizadegan, Kamran |
| collection | MIT |
| description | The use of coherent light in conventional holographic phase
microscopy (HPM) poses three major drawbacks: poor spatial resolution,
weak depth sectioning, and fixed pattern noise due to unwanted diffraction.
Here, we report a technique which can overcome these drawbacks, but
maintains the advantage of phase microscopy - high contrast live cell
imaging and 3D imaging. A speckle beam of a complex spatial pattern is
used for illumination to reduce fixed pattern noise and to improve optical
sectioning capability. By recording of the electric field of speckle, we
demonstrate high contrast 3D live cell imaging without the need for axial
scanning - neither objective lens nor sample stage. This technique has great
potential in studying biological samples with improved sensitivity,
resolution and optical sectioning capability. |
| first_indexed | 2024-09-23T13:37:45Z |
| format | Article |
| id | mit-1721.1/54778 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:37:45Z |
| publishDate | 2010 |
| publisher | Optical Society of America |
| record_format | dspace |
| spelling | mit-1721.1/547782022-09-28T15:10:13Z Speckle-field digital holographic microscopy Badizadegan, Kamran Dasari, Ramachandra Rao Yaqoob, Zahid Choi, Wonshik Feld, Michael S. Park, YongKeun Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Spectroscopy Laboratory Feld, Michael S. Badizadegan, Kamran Dasari, Ramachandra Rao Choi, Wonshik Yaqoob, Zahid Feld, Michael S. Park, YongKeun The use of coherent light in conventional holographic phase microscopy (HPM) poses three major drawbacks: poor spatial resolution, weak depth sectioning, and fixed pattern noise due to unwanted diffraction. Here, we report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging. A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrate high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability. National Science Foundation (DBI-0754339) National Institutes of Health. National Center for Research Resources (P41-RR02594) 2010-05-12T20:47:04Z 2010-05-12T20:47:04Z 2009-07 2009-06 Article http://purl.org/eprint/type/SubmittedJournalArticle 1094-4087 http://hdl.handle.net/1721.1/54778 Park,YongKeun et al. "Speckle-field digital holographic microscopy," Opt. Express 17, 12285-12292 (2009) ©2009 Optical Society of America. en_US http://dx.doi.org/10.1364/OE.17.012285 Optics Express Attribution-Noncommercial-Share Alike 3.0 Unported http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Optical Society of America YongKeun Park |
| spellingShingle | Badizadegan, Kamran Dasari, Ramachandra Rao Yaqoob, Zahid Choi, Wonshik Feld, Michael S. Park, YongKeun Speckle-field digital holographic microscopy |
| title | Speckle-field digital holographic microscopy |
| title_full | Speckle-field digital holographic microscopy |
| title_fullStr | Speckle-field digital holographic microscopy |
| title_full_unstemmed | Speckle-field digital holographic microscopy |
| title_short | Speckle-field digital holographic microscopy |
| title_sort | speckle field digital holographic microscopy |
| url | http://hdl.handle.net/1721.1/54778 |
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