Three-Dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation
Laser scanning systems for two-photon microscopy and fabrication have been proven to be excellent in depth-resolving capability for years. However, their applications have been limited to laboratory use due to their intrinsic slow nature. The recently introduced temporal focusing concept enables wi...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Society of Photo-optical Instrumentation Engineers
2010
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| Online Access: | http://hdl.handle.net/1721.1/54817 https://orcid.org/0000-0003-4698-6488 |
| _version_ | 1811086050397782016 |
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| author | Kim, Daekeun So, Peter T. C. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Kim, Daekeun So, Peter T. C. |
| author_sort | Kim, Daekeun |
| collection | MIT |
| description | Laser scanning systems for two-photon microscopy and fabrication have been proven to be excellent in depth-resolving capability for years. However, their applications have been limited to laboratory use due to their intrinsic slow nature. The recently introduced temporal focusing concept enables wide-field optical sectioning and thus has potential in both high-speed 3D imaging and 3D mass-production fields. In this paper, we use the ultrafast optical pulse manipulation to generate two-photon excitation depth-resolved wide-field illumination (TPEDRWFI). The design parameters for the illumination were chosen based on numerical simulation of the temporal focusing. The imaging system was implemented, and the optical sectioning performance was compared with experimental result. |
| first_indexed | 2024-09-23T13:20:06Z |
| format | Article |
| id | mit-1721.1/54817 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:20:06Z |
| publishDate | 2010 |
| publisher | Society of Photo-optical Instrumentation Engineers |
| record_format | dspace |
| spelling | mit-1721.1/548172022-09-28T13:28:14Z Three-Dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation Kim, Daekeun So, Peter T. C. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering So, Peter T. C. Kim, Daekeun So, Peter T. C. Laser scanning systems for two-photon microscopy and fabrication have been proven to be excellent in depth-resolving capability for years. However, their applications have been limited to laboratory use due to their intrinsic slow nature. The recently introduced temporal focusing concept enables wide-field optical sectioning and thus has potential in both high-speed 3D imaging and 3D mass-production fields. In this paper, we use the ultrafast optical pulse manipulation to generate two-photon excitation depth-resolved wide-field illumination (TPEDRWFI). The design parameters for the illumination were chosen based on numerical simulation of the temporal focusing. The imaging system was implemented, and the optical sectioning performance was compared with experimental result. Deshpande Center for Technological Innovation 2010-05-20T18:13:45Z 2010-05-20T18:13:45Z 2009-02 2009-01 Article http://purl.org/eprint/type/JournalArticle 9780819474292 1605-7422 http://hdl.handle.net/1721.1/54817 Kim, Daekeun, and Peter T. C. So. “Three-dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation.” Multiphoton Microscopy in the Biomedical Sciences IX. Ed. Ammasi Periasamy & Peter T. C. So. San Jose, CA, USA: SPIE, 2009. 71831B-8. © 2009 Society of Photo-optical Instrumentation Engineers. https://orcid.org/0000-0003-4698-6488 en_US http://dx.doi.org/10.1117/12.810910 Proceedings of SPIE--the International Society for Optical Engineering Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Society of Photo-optical Instrumentation Engineers SPIE |
| spellingShingle | Kim, Daekeun So, Peter T. C. Three-Dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation |
| title | Three-Dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation |
| title_full | Three-Dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation |
| title_fullStr | Three-Dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation |
| title_full_unstemmed | Three-Dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation |
| title_short | Three-Dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation |
| title_sort | three dimensional 3d high speed imaging and fabrication system based on ultrafast optical pulse manipulation |
| url | http://hdl.handle.net/1721.1/54817 https://orcid.org/0000-0003-4698-6488 |
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