Photoelectrochemical synthesis of DNA microarrays
Optical addressing of semiconductor electrodes represents a powerful technology that enables the independent and parallel control of a very large number of electrical phenomena at the solid-electrolyte interface. To date, it has been used in a wide range of applications including electrophoretic man...
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| Format: | Article |
| Language: | en_US |
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United States National Academy of Sciences
2010
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| Online Access: | http://hdl.handle.net/1721.1/55288 https://orcid.org/0000-0002-1332-3197 |
| _version_ | 1811070383150858240 |
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| author | Jacobson, Joseph Emig, Christopher J. Chow, Brian Y. |
| author2 | Massachusetts Institute of Technology. Center for Bits and Atoms |
| author_facet | Massachusetts Institute of Technology. Center for Bits and Atoms Jacobson, Joseph Emig, Christopher J. Chow, Brian Y. |
| author_sort | Jacobson, Joseph |
| collection | MIT |
| description | Optical addressing of semiconductor electrodes represents a powerful technology that enables the independent and parallel control of a very large number of electrical phenomena at the solid-electrolyte interface. To date, it has been used in a wide range of applications including electrophoretic manipulation, biomolecule sensing, and stimulating networks of neurons. Here, we have adapted this approach for the parallel addressing of redox reactions, and report the construction of a DNA microarray synthesis platform based on semiconductor photoelectrochemistry (PEC). An amorphous silicon photoconductor is activated by an optical projection system to create virtual electrodes capable of electrochemically generating protons; these PEC-generated protons then cleave the acid-labile dimethoxytrityl protecting groups of DNA phosphoramidite synthesis reagents with the requisite spatial selectivity to generate DNA microarrays. Furthermore, a thin-film porous glass dramatically increases the amount of DNA synthesized per chip by over an order of magnitude versus uncoated glass. This platform demonstrates that PEC can be used toward combinatorial bio-polymer and small molecule synthesis. |
| first_indexed | 2024-09-23T08:35:06Z |
| format | Article |
| id | mit-1721.1/55288 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:35:06Z |
| publishDate | 2010 |
| publisher | United States National Academy of Sciences |
| record_format | dspace |
| spelling | mit-1721.1/552882022-09-23T13:03:55Z Photoelectrochemical synthesis of DNA microarrays Jacobson, Joseph Emig, Christopher J. Chow, Brian Y. Massachusetts Institute of Technology. Center for Bits and Atoms Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Media Laboratory Program in Media Arts and Sciences (Massachusetts Institute of Technology) Jacobson, Joseph Jacobson, Joseph Emig, Christopher J. Chow, Brian Y. Optical addressing of semiconductor electrodes represents a powerful technology that enables the independent and parallel control of a very large number of electrical phenomena at the solid-electrolyte interface. To date, it has been used in a wide range of applications including electrophoretic manipulation, biomolecule sensing, and stimulating networks of neurons. Here, we have adapted this approach for the parallel addressing of redox reactions, and report the construction of a DNA microarray synthesis platform based on semiconductor photoelectrochemistry (PEC). An amorphous silicon photoconductor is activated by an optical projection system to create virtual electrodes capable of electrochemically generating protons; these PEC-generated protons then cleave the acid-labile dimethoxytrityl protecting groups of DNA phosphoramidite synthesis reagents with the requisite spatial selectivity to generate DNA microarrays. Furthermore, a thin-film porous glass dramatically increases the amount of DNA synthesized per chip by over an order of magnitude versus uncoated glass. This platform demonstrates that PEC can be used toward combinatorial bio-polymer and small molecule synthesis. Defense Advanced Research Projects Agency (N66001-05-X6030) National Science Foundation (Grant CCR0122419) 2010-05-26T15:55:30Z 2010-05-26T15:55:30Z 2009-08 2009-06 Article http://purl.org/eprint/type/JournalArticle 1091-6490 0027-8424 http://hdl.handle.net/1721.1/55288 Chow, Brian Y, Christopher J Emig, and Joseph M Jacobson. “Photoelectrochemical synthesis of DNA microarrays.” Proceedings of the National Academy of Sciences 106.36 (2009): 15219-15224. © 2009 National Academy of Sciences https://orcid.org/0000-0002-1332-3197 en_US http://dx.doi.org/10.1073/pnas.0813011106 Proceedings of the National Academy of Sciences of the United States of America Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf United States National Academy of Sciences PNAS |
| spellingShingle | Jacobson, Joseph Emig, Christopher J. Chow, Brian Y. Photoelectrochemical synthesis of DNA microarrays |
| title | Photoelectrochemical synthesis of DNA microarrays |
| title_full | Photoelectrochemical synthesis of DNA microarrays |
| title_fullStr | Photoelectrochemical synthesis of DNA microarrays |
| title_full_unstemmed | Photoelectrochemical synthesis of DNA microarrays |
| title_short | Photoelectrochemical synthesis of DNA microarrays |
| title_sort | photoelectrochemical synthesis of dna microarrays |
| url | http://hdl.handle.net/1721.1/55288 https://orcid.org/0000-0002-1332-3197 |
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