Dynamics of two phosphorelays controlling cell cycle progression in 1 Caulobacter crescentus
In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its pr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en_US |
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American Society for Microbiology
2010
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| Online Access: | http://hdl.handle.net/1721.1/56288 https://orcid.org/0000-0002-8288-7607 |
| _version_ | 1811075422567268352 |
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| author | Chen, Y. Erin Tsokos, Christos G. Biondi, Emanuele G. Perchuk, Barrett Laub, Michael T |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Chen, Y. Erin Tsokos, Christos G. Biondi, Emanuele G. Perchuk, Barrett Laub, Michael T |
| author_sort | Chen, Y. Erin |
| collection | MIT |
| description | In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its proteolysis. Here, we examined whether the CckA phosphorelays also influence the downregulation of CtrA. We demonstrate that CckA is bifunctional, capable of acting as either a kinase or phosphatase to drive the activation or inactivation, respectively, of CtrA. By identifying mutations that uncouple these two activities, we show that CckA's phosphatase activity is important for downregulating CtrA prior to DNA replication initiation in vivo but that other phosphatases may exist. Our results demonstrate that cell cycle transitions in Caulobacter require and are likely driven by the toggling of CckA between its kinase and phosphatase states. More generally, our results emphasize how the bifunctional nature of histidine kinases can help switch cells between mutually exclusive states. |
| first_indexed | 2024-09-23T10:05:38Z |
| format | Article |
| id | mit-1721.1/56288 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:05:38Z |
| publishDate | 2010 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | mit-1721.1/562882022-10-25T04:41:47Z Dynamics of two phosphorelays controlling cell cycle progression in 1 Caulobacter crescentus Chen, Y. Erin Tsokos, Christos G. Biondi, Emanuele G. Perchuk, Barrett Laub, Michael T Massachusetts Institute of Technology. Department of Biology Laub, Michael T. Laub, Michael T. Perchuk, Barrett Tsokos, Christos G. Chen, Y. Erin Biondi, Emanuele G. In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its proteolysis. Here, we examined whether the CckA phosphorelays also influence the downregulation of CtrA. We demonstrate that CckA is bifunctional, capable of acting as either a kinase or phosphatase to drive the activation or inactivation, respectively, of CtrA. By identifying mutations that uncouple these two activities, we show that CckA's phosphatase activity is important for downregulating CtrA prior to DNA replication initiation in vivo but that other phosphatases may exist. Our results demonstrate that cell cycle transitions in Caulobacter require and are likely driven by the toggling of CckA between its kinase and phosphatase states. More generally, our results emphasize how the bifunctional nature of histidine kinases can help switch cells between mutually exclusive states. 2010-07-14T14:31:22Z 2010-07-14T14:31:22Z 2009-09 2009-07 Article http://purl.org/eprint/type/SubmittedJournalArticle 0021-9193 http://hdl.handle.net/1721.1/56288 Chen, Y. Erin et al. “Dynamics of Two Phosphorelays Controlling Cell Cycle Progression in Caulobacter crescentus.” The Journal of Bacteriology 191.24 (2009): 7417-7429. © 2009 Journal of Bacteriology. https://orcid.org/0000-0002-8288-7607 en_US http://dx.doi.org/10.1128/JB.00992-09 Journal of Bacteriology Attribution-Noncommercial-Share Alike 3.0 Unported http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf American Society for Microbiology Michael Laub |
| spellingShingle | Chen, Y. Erin Tsokos, Christos G. Biondi, Emanuele G. Perchuk, Barrett Laub, Michael T Dynamics of two phosphorelays controlling cell cycle progression in 1 Caulobacter crescentus |
| title | Dynamics of two phosphorelays controlling cell cycle progression in 1 Caulobacter crescentus |
| title_full | Dynamics of two phosphorelays controlling cell cycle progression in 1 Caulobacter crescentus |
| title_fullStr | Dynamics of two phosphorelays controlling cell cycle progression in 1 Caulobacter crescentus |
| title_full_unstemmed | Dynamics of two phosphorelays controlling cell cycle progression in 1 Caulobacter crescentus |
| title_short | Dynamics of two phosphorelays controlling cell cycle progression in 1 Caulobacter crescentus |
| title_sort | dynamics of two phosphorelays controlling cell cycle progression in 1 caulobacter crescentus |
| url | http://hdl.handle.net/1721.1/56288 https://orcid.org/0000-0002-8288-7607 |
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