Rapid Biocompatibility Analysis of Materials via In Vivo Fluorescence Imaging of Mouse Models
Background: Many materials are unsuitable for medical use because of poor biocompatibility. Recently, advances in the high throughput synthesis of biomaterials has significantly increased the number of potential biomaterials, however current biocompatibility analysis methods are slow and require h...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Public Library of Science
2010
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| Online Access: | http://hdl.handle.net/1721.1/57466 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 |
| _version_ | 1811070344752005120 |
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| author | Bratlie, Kaitlin M. Dang, Tram T. Lyle, Stephen Nahrendorf, Matthias Weissleder, Ralph Langer, Robert Anderson, Daniel G. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Bratlie, Kaitlin M. Dang, Tram T. Lyle, Stephen Nahrendorf, Matthias Weissleder, Ralph Langer, Robert Anderson, Daniel G. |
| author_sort | Bratlie, Kaitlin M. |
| collection | MIT |
| description | Background:
Many materials are unsuitable for medical use because of poor biocompatibility. Recently, advances in the high throughput synthesis of biomaterials has significantly increased the number of potential biomaterials, however current biocompatibility analysis methods are slow and require histological analysis.
Methodology/Principal Findings:
Here we develop rapid, non-invasive methods for in vivo quantification of the inflammatory response to implanted biomaterials. Materials were placed subcutaneously in an array format and monitored for host responses as per ISO 10993-6: 2001. Host cell activity in response to these materials was imaged kinetically, in vivo using fluorescent whole animal imaging. Data captured using whole animal imaging displayed similar temporal trends in cellular recruitment of phagocytes to the biomaterials compared to histological analysis.
Conclusions/Significance:
Histological analysis similarity validates this technique as a novel, rapid approach for screening biocompatibility of implanted materials. Through this technique there exists the possibility to rapidly screen large libraries of polymers in vivo. |
| first_indexed | 2024-09-23T08:34:28Z |
| format | Article |
| id | mit-1721.1/57466 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:34:28Z |
| publishDate | 2010 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/574662022-09-23T12:58:48Z Rapid Biocompatibility Analysis of Materials via In Vivo Fluorescence Imaging of Mouse Models Bratlie, Kaitlin M. Dang, Tram T. Lyle, Stephen Nahrendorf, Matthias Weissleder, Ralph Langer, Robert Anderson, Daniel G. Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Anderson, Daniel G. Bratlie, Kaitlin M. Dang, Tram T. Langer, Robert Anderson, Daniel Griffith Background: Many materials are unsuitable for medical use because of poor biocompatibility. Recently, advances in the high throughput synthesis of biomaterials has significantly increased the number of potential biomaterials, however current biocompatibility analysis methods are slow and require histological analysis. Methodology/Principal Findings: Here we develop rapid, non-invasive methods for in vivo quantification of the inflammatory response to implanted biomaterials. Materials were placed subcutaneously in an array format and monitored for host responses as per ISO 10993-6: 2001. Host cell activity in response to these materials was imaged kinetically, in vivo using fluorescent whole animal imaging. Data captured using whole animal imaging displayed similar temporal trends in cellular recruitment of phagocytes to the biomaterials compared to histological analysis. Conclusions/Significance: Histological analysis similarity validates this technique as a novel, rapid approach for screening biocompatibility of implanted materials. Through this technique there exists the possibility to rapidly screen large libraries of polymers in vivo. Juvenile Diabetes Research Foundation (grant 17-2007-1063) 2010-08-04T13:25:45Z 2010-08-04T13:25:45Z 2010-04 2010-01 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/57466 Bratlie KM, Dang TT, Lyle S, Nahrendorf M, Weissleder R, et al. 2010 Rapid Biocompatibility Analysis of Materials via In Vivo Fluorescence Imaging of Mouse Models. PLoS ONE 5(4): e10032. doi:10.1371/journal.pone.0010032 20386609 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 en_US http://dx.doi.org/10.1371/journal.pone.0010032 PLoS One Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Bratlie, Kaitlin M. Dang, Tram T. Lyle, Stephen Nahrendorf, Matthias Weissleder, Ralph Langer, Robert Anderson, Daniel G. Rapid Biocompatibility Analysis of Materials via In Vivo Fluorescence Imaging of Mouse Models |
| title | Rapid Biocompatibility Analysis of Materials via In Vivo Fluorescence Imaging of Mouse Models |
| title_full | Rapid Biocompatibility Analysis of Materials via In Vivo Fluorescence Imaging of Mouse Models |
| title_fullStr | Rapid Biocompatibility Analysis of Materials via In Vivo Fluorescence Imaging of Mouse Models |
| title_full_unstemmed | Rapid Biocompatibility Analysis of Materials via In Vivo Fluorescence Imaging of Mouse Models |
| title_short | Rapid Biocompatibility Analysis of Materials via In Vivo Fluorescence Imaging of Mouse Models |
| title_sort | rapid biocompatibility analysis of materials via in vivo fluorescence imaging of mouse models |
| url | http://hdl.handle.net/1721.1/57466 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 |
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