Ecology and population structure of vibrionaceae in the coastal ocean
Thesis (Ph.D.)--Joint Program in Oceanography (Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering; and the Woods Hole Oceanographic Institution), 2010.
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
| Published: |
Massachusetts Institute of Technology
2010
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/1721.1/58184 |
| _version_ | 1811085200798515200 |
|---|---|
| author | Preheim, Sarah Pacocha |
| author2 | Martin F. Polz. |
| author_facet | Martin F. Polz. Preheim, Sarah Pacocha |
| author_sort | Preheim, Sarah Pacocha |
| collection | MIT |
| description | Thesis (Ph.D.)--Joint Program in Oceanography (Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering; and the Woods Hole Oceanographic Institution), 2010. |
| first_indexed | 2024-09-23T13:05:14Z |
| format | Thesis |
| id | mit-1721.1/58184 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T13:05:14Z |
| publishDate | 2010 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/581842022-01-12T18:39:19Z Ecology and population structure of vibrionaceae in the coastal ocean Preheim, Sarah Pacocha Martin F. Polz. Woods Hole Oceanographic Institution. Joint Program in Oceanography Massachusetts Institute of Technology. Dept. of Civil and Environmental Engineering. Woods Hole Oceanographic Institution. Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Joint Program in Oceanography. Civil and Environmental Engineering. Woods Hole Oceanographic Institution. Marine microbiology Microbial diversity Thesis (Ph.D.)--Joint Program in Oceanography (Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering; and the Woods Hole Oceanographic Institution), 2010. Cataloged from PDF version of thesis. Includes bibliographical references. Extensive genetic diversity has been discovered in the microbial world, yet mechanisms that shape and maintain this diversity remain poorly understood. This thesis investigates to what extent populations of the gamma-proteobacterial family, Vibrionaceae, are ecologically specialized by investigating the distribution across a wide range of environmental categories, such as marine invertebrates or particles in the water column. Additionally, it seeks to determine whether in situ population distributions directly result from a competitive advantage over other Vibrio populations. This was investigated by in vitro competition assays on mixtures of native, sterilized particles. Generalist populations were found to dominate the associations with marine invertebrates, consistent with a model of high migration dominated population assembly. A majority of populations occurred broadly within and among the different types of invertebrates sampled, with one population being a near perfect generalist with regard to seasons, host taxa and body regions. High variability across host individuals, consistent with a scenario of stochastic clonal expansion, was especially pronounced in crab and zooplankton samples. Specialization, demonstrated by specific and reproducible association with different particle types in the water column, is more common than specialization within invertebrate hosts. (cont.) Co-existing Vibrio species show strong preferences for different types of particulate matter in the water column suggesting that competition for limited resources influences their evolution. While populations show different growth profiles on particle derived substrates, relative growth advantages of specialist populations in competition with other Vibrio populations on native particles may not be sufficient to explain observed environmental distributions. Instead, populations may gain an advantage on these particles by colonizing the living plant or zooplankton prior to death and degradation into particulate matter. In summary, although vibrios are known commensals of marine invertebrates, evidence suggests that population structure within animals is fairly weak compared to suspended particles in the water column. This highlights the importance of comparing multiple environmental categories and migration among them to investigate population structure and adaptation. by Sarah Pacocha Preheim. Ph.D. 2010-09-02T14:56:01Z 2010-09-02T14:56:01Z 2010 2010 Thesis http://hdl.handle.net/1721.1/58184 639534801 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 183 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Joint Program in Oceanography. Civil and Environmental Engineering. Woods Hole Oceanographic Institution. Marine microbiology Microbial diversity Preheim, Sarah Pacocha Ecology and population structure of vibrionaceae in the coastal ocean |
| title | Ecology and population structure of vibrionaceae in the coastal ocean |
| title_full | Ecology and population structure of vibrionaceae in the coastal ocean |
| title_fullStr | Ecology and population structure of vibrionaceae in the coastal ocean |
| title_full_unstemmed | Ecology and population structure of vibrionaceae in the coastal ocean |
| title_short | Ecology and population structure of vibrionaceae in the coastal ocean |
| title_sort | ecology and population structure of vibrionaceae in the coastal ocean |
| topic | Joint Program in Oceanography. Civil and Environmental Engineering. Woods Hole Oceanographic Institution. Marine microbiology Microbial diversity |
| url | http://hdl.handle.net/1721.1/58184 |
| work_keys_str_mv | AT preheimsarahpacocha ecologyandpopulationstructureofvibrionaceaeinthecoastalocean |