Dazl regulates mouse embryonic germ cell development
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2010.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2010
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| Online Access: | http://hdl.handle.net/1721.1/58372 |
| _version_ | 1811097476719968256 |
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| author | Gill, Mark E |
| author2 | David C. Page. |
| author_facet | David C. Page. Gill, Mark E |
| author_sort | Gill, Mark E |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2010. |
| first_indexed | 2024-09-23T17:00:06Z |
| format | Thesis |
| id | mit-1721.1/58372 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T17:00:06Z |
| publishDate | 2010 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/583722019-04-12T12:13:08Z Dazl regulates mouse embryonic germ cell development Gill, Mark E David C. Page. Massachusetts Institute of Technology. Dept. of Biology. Massachusetts Institute of Technology. Dept. of Biology. Biology. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2010. Cataloged from PDF version of thesis. Includes bibliographical references. In the mouse, germ cells can undergo differentiation to become either oocytes or spermatozoa in response to sex of their gonadal environment. The nature of the germ cell-intrinsic aspects of this signaling have not been well studied. The earliest known sex-specific difference in germ cells is the initiation of meiosis in female, but not male, embryonic germ cells. Experiments were performed showing that germ cells of both sexes transit through a state, the meiosis competent germ cell, that is required for initiation of meiosis. Acquisition of this state requires the function of the germ cellspecific RNA binding protein DAZL. The sufficiency for the absence of meiosis to drive male germ cell differentiation was then tested by examining non-meiotic XX germ cells in the Dazl-deficient ovary. These cells did not exhibit male differentiation indicating that the absence of meiosis is not sufficient for male differentiation. XX Dazl-deficient germ cells also failed to exhibit normal female differentiation. In addition, XY Dazl-deficient germ cells do not display characteristics of either male or female germ cells. Taken together, these results indicate that germ cells must first undergo a sex non-specific differentiation step prior to acquiring sexual fate. by Mark E. Gill. Ph.D. 2010-09-03T18:29:34Z 2010-09-03T18:29:34Z 2010 2010 Thesis http://hdl.handle.net/1721.1/58372 613396440 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 134 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biology. Gill, Mark E Dazl regulates mouse embryonic germ cell development |
| title | Dazl regulates mouse embryonic germ cell development |
| title_full | Dazl regulates mouse embryonic germ cell development |
| title_fullStr | Dazl regulates mouse embryonic germ cell development |
| title_full_unstemmed | Dazl regulates mouse embryonic germ cell development |
| title_short | Dazl regulates mouse embryonic germ cell development |
| title_sort | dazl regulates mouse embryonic germ cell development |
| topic | Biology. |
| url | http://hdl.handle.net/1721.1/58372 |
| work_keys_str_mv | AT gillmarke dazlregulatesmouseembryonicgermcelldevelopment |