High-throughput Microfluidics and Ultrafast Optics for in Vivo Compound/Genetic Discoveries
Therapeutic treatment of spinal cord injuries, brain trauma, stroke, and neurodegenerative diseases will greatly benefit from the discovery of compounds that enhance neuronal regeneration following injury. We previously demonstrated the use of femtosecond laser microsurgery to induce precise and rep...
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| Format: | Article |
| Language: | en_US |
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SPIE
2010
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| Online Access: | http://hdl.handle.net/1721.1/58554 https://orcid.org/0000-0002-4612-1962 |
| _version_ | 1826213613997654016 |
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| author | Rohde, Christopher Benjamin Gilleland, Cody Lee Samara, Chrysanthi Yanik, Mehmet Fatih |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Rohde, Christopher Benjamin Gilleland, Cody Lee Samara, Chrysanthi Yanik, Mehmet Fatih |
| author_sort | Rohde, Christopher Benjamin |
| collection | MIT |
| description | Therapeutic treatment of spinal cord injuries, brain trauma, stroke, and neurodegenerative diseases will greatly benefit from the discovery of compounds that enhance neuronal regeneration following injury. We previously demonstrated the use of femtosecond laser microsurgery to induce precise and reproducible neural injury in C. elegans, and have developed microfluidic on-chip technologies that allow automated and rapid manipulation, orientation, and non-invasive immobilization of animals for sub-cellular resolution two-photon imaging and femtosecond-laser nanosurgery. These technologies include microfluidic whole-animal sorters, as well as integrated chips containing multiple addressable incubation chambers for exposure of individual animals to compounds and sub-cellular time-lapse imaging of hundreds of animals on a single chip. Our technologies can be used for a variety of highly sophisticated in vivo high-throughput compound and genetic screens, and we performed the first in vivo screen in C. elegans for compounds enhancing neuronal regrowth following femtosecond microsurgery. The compounds identified interact with a wide variety of cellular targets, such as cytoskeletal components, vesicle trafficking, and protein kinases that enhance neuronal regeneration. |
| first_indexed | 2024-09-23T15:52:03Z |
| format | Article |
| id | mit-1721.1/58554 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:52:03Z |
| publishDate | 2010 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/585542022-10-02T04:43:14Z High-throughput Microfluidics and Ultrafast Optics for in Vivo Compound/Genetic Discoveries Rohde, Christopher Benjamin Gilleland, Cody Lee Samara, Chrysanthi Yanik, Mehmet Fatih Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Yanik, Mehmet Fatih Rohde, Christopher Benjamin Gilleland, Cody Lee Samara, Chrysanthi Yanik, Mehmet Fatih Femtosecond laser surgery neural regeneration C. elegans microfluidics high-throughput Therapeutic treatment of spinal cord injuries, brain trauma, stroke, and neurodegenerative diseases will greatly benefit from the discovery of compounds that enhance neuronal regeneration following injury. We previously demonstrated the use of femtosecond laser microsurgery to induce precise and reproducible neural injury in C. elegans, and have developed microfluidic on-chip technologies that allow automated and rapid manipulation, orientation, and non-invasive immobilization of animals for sub-cellular resolution two-photon imaging and femtosecond-laser nanosurgery. These technologies include microfluidic whole-animal sorters, as well as integrated chips containing multiple addressable incubation chambers for exposure of individual animals to compounds and sub-cellular time-lapse imaging of hundreds of animals on a single chip. Our technologies can be used for a variety of highly sophisticated in vivo high-throughput compound and genetic screens, and we performed the first in vivo screen in C. elegans for compounds enhancing neuronal regrowth following femtosecond microsurgery. The compounds identified interact with a wide variety of cellular targets, such as cytoskeletal components, vesicle trafficking, and protein kinases that enhance neuronal regeneration. 2010-09-15T18:55:16Z 2010-09-15T18:55:16Z 2010-02 2010-01 Article http://purl.org/eprint/type/JournalArticle 0277-786X http://hdl.handle.net/1721.1/58554 Rohde, Christopher B. et al. “High-throughput microfluidics and ultrafast optics for in vivo compound/genetic discoveries.” Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII. Ed. Daniel L. Farkas, Dan V. Nicolau, & Robert C. Leif. San Francisco, California, USA: SPIE, 2010. 75680N-6. ©2010 COPYRIGHT SPIE--The International Society for Optical Engineering. https://orcid.org/0000-0002-4612-1962 en_US http://dx.doi.org/10.1117/12.841748 Proceedings of SPIE--the International Society for Optical Engineering; v. 7568 Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Femtosecond laser surgery neural regeneration C. elegans microfluidics high-throughput Rohde, Christopher Benjamin Gilleland, Cody Lee Samara, Chrysanthi Yanik, Mehmet Fatih High-throughput Microfluidics and Ultrafast Optics for in Vivo Compound/Genetic Discoveries |
| title | High-throughput Microfluidics and Ultrafast Optics for in Vivo Compound/Genetic Discoveries |
| title_full | High-throughput Microfluidics and Ultrafast Optics for in Vivo Compound/Genetic Discoveries |
| title_fullStr | High-throughput Microfluidics and Ultrafast Optics for in Vivo Compound/Genetic Discoveries |
| title_full_unstemmed | High-throughput Microfluidics and Ultrafast Optics for in Vivo Compound/Genetic Discoveries |
| title_short | High-throughput Microfluidics and Ultrafast Optics for in Vivo Compound/Genetic Discoveries |
| title_sort | high throughput microfluidics and ultrafast optics for in vivo compound genetic discoveries |
| topic | Femtosecond laser surgery neural regeneration C. elegans microfluidics high-throughput |
| url | http://hdl.handle.net/1721.1/58554 https://orcid.org/0000-0002-4612-1962 |
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