Simultaneous imaging of GFP, CFP and collagen in tumors in vivousing multiphoton microscopy
Background: The development of multiphoton laser scanning microscopy has greatly facilitated the imaging of living tissues. However, the use of genetically encoded fluorescent proteins to distinguish different cell types in living animals has not been described at single cell resolution using multip...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
BioMed Central Ltd
2010
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| Online Access: | http://hdl.handle.net/1721.1/58675 https://orcid.org/0000-0003-3214-4554 |
| _version_ | 1826203426602614784 |
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| author | Sahai, Erik Wyckoff, Jeffrey Philippar, Ulrike Segall, Jeffrey E Gertler, Frank Condeelis, John S. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Sahai, Erik Wyckoff, Jeffrey Philippar, Ulrike Segall, Jeffrey E Gertler, Frank Condeelis, John S. |
| author_sort | Sahai, Erik |
| collection | MIT |
| description | Background: The development of multiphoton laser scanning microscopy has greatly facilitated the imaging of living tissues. However, the use of genetically encoded fluorescent proteins to distinguish different cell types in living animals has not been described at single cell resolution using multiphoton microscopy. Results: Here we describe a method for the simultaneous imaging, by multiphoton microscopy, of Green Fluorescent Protein, Cyan Fluorescent Protein and collagen in vivo in living tumors. This novel method enables: 1) the simultaneous visualization of overall cell shape and sub-cellular structures such as the plasma membrane or proteins of interest in cells inside living animals, 2) direct comparison of the behavior of single cells from different cell lines in the same microenvironment in vivo. Conclusion: Using this multi-fluor, multiphoton technique, we demonstrate that motility and metastatic differences between carcinoma cells of differing metastatic potential can be imaged in the same animal simultaneously at sub-cellular resolution. |
| first_indexed | 2024-09-23T12:36:28Z |
| format | Article |
| id | mit-1721.1/58675 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:36:28Z |
| publishDate | 2010 |
| publisher | BioMed Central Ltd |
| record_format | dspace |
| spelling | mit-1721.1/586752022-09-28T08:58:45Z Simultaneous imaging of GFP, CFP and collagen in tumors in vivousing multiphoton microscopy Sahai, Erik Wyckoff, Jeffrey Philippar, Ulrike Segall, Jeffrey E Gertler, Frank Condeelis, John S. Massachusetts Institute of Technology. Department of Biology Philippar, Ulrike Gertler, Frank Background: The development of multiphoton laser scanning microscopy has greatly facilitated the imaging of living tissues. However, the use of genetically encoded fluorescent proteins to distinguish different cell types in living animals has not been described at single cell resolution using multiphoton microscopy. Results: Here we describe a method for the simultaneous imaging, by multiphoton microscopy, of Green Fluorescent Protein, Cyan Fluorescent Protein and collagen in vivo in living tumors. This novel method enables: 1) the simultaneous visualization of overall cell shape and sub-cellular structures such as the plasma membrane or proteins of interest in cells inside living animals, 2) direct comparison of the behavior of single cells from different cell lines in the same microenvironment in vivo. Conclusion: Using this multi-fluor, multiphoton technique, we demonstrate that motility and metastatic differences between carcinoma cells of differing metastatic potential can be imaged in the same animal simultaneously at sub-cellular resolution. National Institutes of Health (U.S) ( grant CA100324) National Institutes of Health (U.S) ( grant GM58801) International Union against Cancer (Aventis Translational Cancer Research Fellowship) 2010-09-23T12:59:02Z 2010-09-23T12:59:02Z 2005-05 2005-02 2010-09-03T16:23:21Z Article http://purl.org/eprint/type/JournalArticle 1472-6750 http://hdl.handle.net/1721.1/58675 BMC Biotechnology. 2005 May 23;5(1):14 15910685 https://orcid.org/0000-0003-3214-4554 en http://dx.doi.org/10.1186/1472-6750-5-14 BMC Biotechnology Creative Commons Attribution http://creativecommons.org/licenses/by/2.0 Sahai et al.; licensee BioMed Central Ltd. application/pdf BioMed Central Ltd BioMed Central Ltd |
| spellingShingle | Sahai, Erik Wyckoff, Jeffrey Philippar, Ulrike Segall, Jeffrey E Gertler, Frank Condeelis, John S. Simultaneous imaging of GFP, CFP and collagen in tumors in vivousing multiphoton microscopy |
| title | Simultaneous imaging of GFP, CFP and collagen in tumors in vivousing multiphoton microscopy |
| title_full | Simultaneous imaging of GFP, CFP and collagen in tumors in vivousing multiphoton microscopy |
| title_fullStr | Simultaneous imaging of GFP, CFP and collagen in tumors in vivousing multiphoton microscopy |
| title_full_unstemmed | Simultaneous imaging of GFP, CFP and collagen in tumors in vivousing multiphoton microscopy |
| title_short | Simultaneous imaging of GFP, CFP and collagen in tumors in vivousing multiphoton microscopy |
| title_sort | simultaneous imaging of gfp cfp and collagen in tumors in vivousing multiphoton microscopy |
| url | http://hdl.handle.net/1721.1/58675 https://orcid.org/0000-0003-3214-4554 |
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