Zebrafish promoter microarrays identify actively transcribed embryonic genes

Zebrafish promoter microarrays identify actively transcribed embryonic genes

We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demon...

Full description

Bibliographic Details
Main Authors: Wardle, Fiona C, Odom, Duncan, Bell, George W., Wiellette, Elizabeth L, Herbolsheimer, Elizabeth, Smith, James C, Yuan, Bingbing B., Sive, Hazel L., Danford, Timothy William, Young, Richard A.
Other Authors: Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Format: Article
Language:English
Published: BioMed Central Ltd 2010
Online Access:http://hdl.handle.net/1721.1/58880
https://orcid.org/0000-0002-4890-424X
https://orcid.org/0000-0001-8855-8647
Description
Summary:We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.

Similar Items