Zebrafish promoter microarrays identify actively transcribed embryonic genes
We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demon...
| Main Authors: | , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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BioMed Central Ltd
2010
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| Online Access: | http://hdl.handle.net/1721.1/58880 https://orcid.org/0000-0002-4890-424X https://orcid.org/0000-0001-8855-8647 |
| _version_ | 1811070818298363904 |
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| author | Wardle, Fiona C Odom, Duncan Bell, George W. Wiellette, Elizabeth L Herbolsheimer, Elizabeth Smith, James C Yuan, Bingbing B. Sive, Hazel L. Danford, Timothy William Young, Richard A. |
| author2 | Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory |
| author_facet | Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory Wardle, Fiona C Odom, Duncan Bell, George W. Wiellette, Elizabeth L Herbolsheimer, Elizabeth Smith, James C Yuan, Bingbing B. Sive, Hazel L. Danford, Timothy William Young, Richard A. |
| author_sort | Wardle, Fiona C |
| collection | MIT |
| description | We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis. |
| first_indexed | 2024-09-23T08:42:05Z |
| format | Article |
| id | mit-1721.1/58880 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:42:05Z |
| publishDate | 2010 |
| publisher | BioMed Central Ltd |
| record_format | dspace |
| spelling | mit-1721.1/588802022-09-23T13:56:06Z Zebrafish promoter microarrays identify actively transcribed embryonic genes Wardle, Fiona C Odom, Duncan Bell, George W. Wiellette, Elizabeth L Herbolsheimer, Elizabeth Smith, James C Yuan, Bingbing B. Sive, Hazel L. Danford, Timothy William Young, Richard A. Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Yuan, Bingbing B. Sive, Hazel L. Danford, Timothy William Young, Richard A. Bell, George W. We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis. Wellcome Trust European Molecular Biology Organization National Institutes of Health (Grant HG002668) (Grant DK070813) (Grant 2RO1 HD33472) (Grant 5RO1 MH59942) 2010-10-05T20:08:23Z 2010-10-05T20:08:23Z 2006-08 2006-04 2010-09-03T16:14:25Z Article http://purl.org/eprint/type/JournalArticle 1474-760X 1465-6914 1465-6906 http://hdl.handle.net/1721.1/58880 Genome Biology. 2006 Aug 04;7(8):R71 16889661 https://orcid.org/0000-0002-4890-424X https://orcid.org/0000-0001-8855-8647 en http://dx.doi.org/10.1186/gb-2006-7-8-r71 Genome Biology Creative Commons Attribution http://creativecommons.org/licenses/by/2.0 Wardle et al.; licensee BioMed Central Ltd. application/pdf BioMed Central Ltd BioMed Central Ltd |
| spellingShingle | Wardle, Fiona C Odom, Duncan Bell, George W. Wiellette, Elizabeth L Herbolsheimer, Elizabeth Smith, James C Yuan, Bingbing B. Sive, Hazel L. Danford, Timothy William Young, Richard A. Zebrafish promoter microarrays identify actively transcribed embryonic genes |
| title | Zebrafish promoter microarrays identify actively transcribed embryonic genes |
| title_full | Zebrafish promoter microarrays identify actively transcribed embryonic genes |
| title_fullStr | Zebrafish promoter microarrays identify actively transcribed embryonic genes |
| title_full_unstemmed | Zebrafish promoter microarrays identify actively transcribed embryonic genes |
| title_short | Zebrafish promoter microarrays identify actively transcribed embryonic genes |
| title_sort | zebrafish promoter microarrays identify actively transcribed embryonic genes |
| url | http://hdl.handle.net/1721.1/58880 https://orcid.org/0000-0002-4890-424X https://orcid.org/0000-0001-8855-8647 |
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