Altering coenzyme specificity of Pichia stipitis xylose reductase by the semi-rational approach CASTing

Background: The NAD(P)H-dependent Pichia stipitis xylose reductase (PsXR) is one of the key enzymes for xylose fermentation, and has been cloned into the commonly used ethanol-producing yeast Saccharomyces cerevisiae. In order to eliminate the redox imbalance resulting from the preference of this en...

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Bibliographic Details
Main Authors: Liang, Ling, Zhang, Jingqing, Lin, Zhanglin
Other Authors: Massachusetts Institute of Technology. Department of Chemical Engineering
Format: Article
Language:English
Published: BioMed Central Ltd 2010
Online Access:http://hdl.handle.net/1721.1/58912