Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice
Background: In mice, germ cells are specified through signalling between layers of cells comprising the primitive embryo. The function of Dppa3 (also known as Pgc7 or stella), a gene expressed in primordial germ cells at the time of their emergence in gastrulating embryos, is unknown, but a recent s...
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| Format: | Article |
| Language: | English |
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BioMed Central Ltd
2010
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| Online Access: | http://hdl.handle.net/1721.1/59309 https://orcid.org/0000-0001-9920-3411 |
| _version_ | 1811005673470689280 |
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| author | Bortvin, Alex Goodheart, Mary L. Liao, Michelle Page, David C. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Bortvin, Alex Goodheart, Mary L. Liao, Michelle Page, David C. |
| author_sort | Bortvin, Alex |
| collection | MIT |
| description | Background: In mice, germ cells are specified through signalling between layers of cells comprising the primitive embryo. The function of Dppa3 (also known as Pgc7 or stella), a gene expressed in primordial germ cells at the time of their emergence in gastrulating embryos, is unknown, but a recent study has claimed that it plays a central role in germ cell specification. Results: To test Dppa3's role in germ cell development, we disrupted the gene in mouse embryonic stem cells and generated mutant animals. We were able to obtain viable and fertile Dppa3-deficient animals of both sexes. Examination of embryonic and adult germ cells and gonads in Dppa3-deficient animals did not reveal any defects. However, most embryos derived from Dppa3-deficient oocytes failed to develop normally beyond the four-cell stage. Conclusion: We found that Dppa3 is an important maternal factor in the cleavage stages of mouse embryogenesis. However, it is not required for germ cell specification. |
| first_indexed | 2024-09-23T16:39:57Z |
| format | Article |
| id | mit-1721.1/59309 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:39:57Z |
| publishDate | 2010 |
| publisher | BioMed Central Ltd |
| record_format | dspace |
| spelling | mit-1721.1/593092022-09-29T20:37:21Z Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice Bortvin, Alex Goodheart, Mary L. Liao, Michelle Page, David C. Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Bortvin, Alex Goodheart, Mary L. Liao, Michelle Page, David C. Animals Biological factors, physiology Cell differentiation, physiology Embryo, mammalian, cytology Embryo, mammalian, physiology Female Gene expression regulation, developmental, physiology Germ cells, physiology Gonads, cytology Gonads, physiology Male Mice Ovary, cytology Ovary, physiology Proteins, physiology Repressor proteins, physiology Biological factors Dppa3 protein, mouse Proteins Repressor proteins Background: In mice, germ cells are specified through signalling between layers of cells comprising the primitive embryo. The function of Dppa3 (also known as Pgc7 or stella), a gene expressed in primordial germ cells at the time of their emergence in gastrulating embryos, is unknown, but a recent study has claimed that it plays a central role in germ cell specification. Results: To test Dppa3's role in germ cell development, we disrupted the gene in mouse embryonic stem cells and generated mutant animals. We were able to obtain viable and fertile Dppa3-deficient animals of both sexes. Examination of embryonic and adult germ cells and gonads in Dppa3-deficient animals did not reveal any defects. However, most embryos derived from Dppa3-deficient oocytes failed to develop normally beyond the four-cell stage. Conclusion: We found that Dppa3 is an important maternal factor in the cleavage stages of mouse embryogenesis. However, it is not required for germ cell specification. Howard Hughes Medical Institute 2010-10-14T12:36:19Z 2010-10-14T12:36:19Z 2004-02 2003-12 2010-09-03T16:01:08Z Article http://purl.org/eprint/type/JournalArticle 1471-213X http://hdl.handle.net/1721.1/59309 Bortvin, Alex, Mary Goodheart, Michelle Liao, and David C. Page (2004). Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice. BMC developmental biology 4:2/1-15. 15018652 https://orcid.org/0000-0001-9920-3411 en http://dx.doi.org/10.1186/1471-213X-4-2 BMC Developmental Biology Creative Commons Attribution Bortvin et al.; licensee BioMed Central Ltd. application/pdf BioMed Central Ltd BioMed Central Ltd |
| spellingShingle | Animals Biological factors, physiology Cell differentiation, physiology Embryo, mammalian, cytology Embryo, mammalian, physiology Female Gene expression regulation, developmental, physiology Germ cells, physiology Gonads, cytology Gonads, physiology Male Mice Ovary, cytology Ovary, physiology Proteins, physiology Repressor proteins, physiology Biological factors Dppa3 protein, mouse Proteins Repressor proteins Bortvin, Alex Goodheart, Mary L. Liao, Michelle Page, David C. Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice |
| title | Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice |
| title_full | Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice |
| title_fullStr | Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice |
| title_full_unstemmed | Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice |
| title_short | Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice |
| title_sort | dppa3 pgc7 stellais a maternal factor and is not required for germ cell specification in mice |
| topic | Animals Biological factors, physiology Cell differentiation, physiology Embryo, mammalian, cytology Embryo, mammalian, physiology Female Gene expression regulation, developmental, physiology Germ cells, physiology Gonads, cytology Gonads, physiology Male Mice Ovary, cytology Ovary, physiology Proteins, physiology Repressor proteins, physiology Biological factors Dppa3 protein, mouse Proteins Repressor proteins |
| url | http://hdl.handle.net/1721.1/59309 https://orcid.org/0000-0001-9920-3411 |
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