A Toggle Involving Cis-Interfering Noncoding RNAs Controls Variegated Gene Expression in Yeast
The identification of specific functional roles for the numerous long noncoding (nc)RNAs found in eukaryotic transcriptomes is currently a matter of intense study amid speculation that these ncRNAs have key regulatory roles. We have identified a pair of cis-interfering ncRNAs in yeast that contribut...
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| Format: | Article |
| Language: | en_US |
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National Academy of Sciences
2010
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| Online Access: | http://hdl.handle.net/1721.1/59327 https://orcid.org/0000-0001-7665-9985 https://orcid.org/0000-0003-3704-2899 https://orcid.org/0000-0003-1709-4034 |
| _version_ | 1826212098885025792 |
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| author | Bumgarner, Stacie L. Dowell, Robin D. Grisafi, Paula Gifford, David K Fink, Gerald R |
| author2 | Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory |
| author_facet | Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory Bumgarner, Stacie L. Dowell, Robin D. Grisafi, Paula Gifford, David K Fink, Gerald R |
| author_sort | Bumgarner, Stacie L. |
| collection | MIT |
| description | The identification of specific functional roles for the numerous long noncoding (nc)RNAs found in eukaryotic transcriptomes is currently a matter of intense study amid speculation that these ncRNAs have key regulatory roles. We have identified a pair of cis-interfering ncRNAs in yeast that contribute to the control of variegated gene expression at the FLO11 locus by implementing a regulatory circuit that toggles between two stable states. These capped, polyadenylated ncRNAs are transcribed across the large intergenic region upstream of the FLO11 ORF. As with mammalian long intervening (li)ncRNAs, these yeast ncRNAs (ICR1 and PWR1) are themselves regulated by transcription factors (Sfl1 and Flo8) and chromatin remodelers (Rpd3L) that are key elements in phenotypic transitions in yeast. The mechanism that we describe explains the unanticipated role of a histone deacetylase complex in activating gene expression, because Rpd3L mutants force the ncRNA circuit into a state that silences the expression of the adjacent variegating gene. |
| first_indexed | 2024-09-23T15:16:22Z |
| format | Article |
| id | mit-1721.1/59327 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:16:22Z |
| publishDate | 2010 |
| publisher | National Academy of Sciences |
| record_format | dspace |
| spelling | mit-1721.1/593272022-09-29T13:48:43Z A Toggle Involving Cis-Interfering Noncoding RNAs Controls Variegated Gene Expression in Yeast Bumgarner, Stacie L. Dowell, Robin D. Grisafi, Paula Gifford, David K Fink, Gerald R Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Whitehead Institute for Biomedical Research Fink, Gerald R. Bumgarner, Stacie L. Dowell, Robin D. Gifford, David K. Fink, Gerald R. The identification of specific functional roles for the numerous long noncoding (nc)RNAs found in eukaryotic transcriptomes is currently a matter of intense study amid speculation that these ncRNAs have key regulatory roles. We have identified a pair of cis-interfering ncRNAs in yeast that contribute to the control of variegated gene expression at the FLO11 locus by implementing a regulatory circuit that toggles between two stable states. These capped, polyadenylated ncRNAs are transcribed across the large intergenic region upstream of the FLO11 ORF. As with mammalian long intervening (li)ncRNAs, these yeast ncRNAs (ICR1 and PWR1) are themselves regulated by transcription factors (Sfl1 and Flo8) and chromatin remodelers (Rpd3L) that are key elements in phenotypic transitions in yeast. The mechanism that we describe explains the unanticipated role of a histone deacetylase complex in activating gene expression, because Rpd3L mutants force the ncRNA circuit into a state that silences the expression of the adjacent variegating gene. 2010-10-14T15:57:40Z 2010-10-14T15:57:40Z 2009-10 2009-08 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/59327 Bumgarner, Stacie L. et al. “Toggle involving cis-interfering noncoding RNAs controls variegated gene expression in yeast.” Proceedings of the National Academy of Sciences 106.43 (2009): 18321 -18326. © 2009 National Academy of Sciences 19805129 https://orcid.org/0000-0001-7665-9985 https://orcid.org/0000-0003-3704-2899 https://orcid.org/0000-0003-1709-4034 en_US http://dx.doi.org/10.1073/pnas.0909641106 Proceedings of the National Academy of Sciences of the United States of America Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences PNAS |
| spellingShingle | Bumgarner, Stacie L. Dowell, Robin D. Grisafi, Paula Gifford, David K Fink, Gerald R A Toggle Involving Cis-Interfering Noncoding RNAs Controls Variegated Gene Expression in Yeast |
| title | A Toggle Involving Cis-Interfering Noncoding RNAs Controls Variegated Gene Expression in Yeast |
| title_full | A Toggle Involving Cis-Interfering Noncoding RNAs Controls Variegated Gene Expression in Yeast |
| title_fullStr | A Toggle Involving Cis-Interfering Noncoding RNAs Controls Variegated Gene Expression in Yeast |
| title_full_unstemmed | A Toggle Involving Cis-Interfering Noncoding RNAs Controls Variegated Gene Expression in Yeast |
| title_short | A Toggle Involving Cis-Interfering Noncoding RNAs Controls Variegated Gene Expression in Yeast |
| title_sort | toggle involving cis interfering noncoding rnas controls variegated gene expression in yeast |
| url | http://hdl.handle.net/1721.1/59327 https://orcid.org/0000-0001-7665-9985 https://orcid.org/0000-0003-3704-2899 https://orcid.org/0000-0003-1709-4034 |
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