Insights into GATA-1 Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis
The transcription factor GATA-1 is required for terminal erythroid maturation and functions as an activator or repressor depending on gene context. Yet its in vivo site selectivity and ability to distinguish between activated versus repressed genes remain incompletely understood. In this study, w...
| Main Authors: | , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier
2010
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| Online Access: | http://hdl.handle.net/1721.1/60029 https://orcid.org/0000-0001-9249-8181 |
| Summary: | The transcription factor GATA-1 is required for terminal erythroid maturation and functions
as an activator or repressor depending on gene context. Yet its in vivo site selectivity and ability
to distinguish between activated versus repressed genes remain incompletely understood. In this
study, we performed GATA-1 ChIP-seq in erythroid cells and compared it to GATA-1 induced
gene expression changes. Bound and differentially expressed genes contain a greater number of
GATA binding motifs, a higher frequency of palindromic GATA sites, and closer occupancy to
the transcriptional start site versus non-differentially expressed genes. Moreover, we show that
the transcription factor Zbtb7a occupies GATA-1 bound regions of some direct GATA-1 target
genes, that the presence of SCL/TAL1 helps distinguish transcriptional activation versus
repression, and that Polycomb Repressive Complex 2 (PRC2) is involved in epigenetic silencing
of a subset of GATA-1 repressed genes. These data provide insights into GATA-1 mediated
gene regulation in vivo. |
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