Insights into GATA-1 Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis
The transcription factor GATA-1 is required for terminal erythroid maturation and functions as an activator or repressor depending on gene context. Yet its in vivo site selectivity and ability to distinguish between activated versus repressed genes remain incompletely understood. In this study, w...
| Main Authors: | , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier
2010
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| Online Access: | http://hdl.handle.net/1721.1/60029 https://orcid.org/0000-0001-9249-8181 |
| _version_ | 1826191680349405184 |
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| author | Yu, Ming Riva, Laura Schindler, Yocheved Moran, Tyler B. Cheng, Yong Yu, Duonan Hardison, Ross C. Weiss, Mitchell J. Orkin, Stuart H. Bernstein, Bradley E. Fraenkel, Ernest Cantor, Alan B. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Yu, Ming Riva, Laura Schindler, Yocheved Moran, Tyler B. Cheng, Yong Yu, Duonan Hardison, Ross C. Weiss, Mitchell J. Orkin, Stuart H. Bernstein, Bradley E. Fraenkel, Ernest Cantor, Alan B. |
| author_sort | Yu, Ming |
| collection | MIT |
| description | The transcription factor GATA-1 is required for terminal erythroid maturation and functions
as an activator or repressor depending on gene context. Yet its in vivo site selectivity and ability
to distinguish between activated versus repressed genes remain incompletely understood. In this
study, we performed GATA-1 ChIP-seq in erythroid cells and compared it to GATA-1 induced
gene expression changes. Bound and differentially expressed genes contain a greater number of
GATA binding motifs, a higher frequency of palindromic GATA sites, and closer occupancy to
the transcriptional start site versus non-differentially expressed genes. Moreover, we show that
the transcription factor Zbtb7a occupies GATA-1 bound regions of some direct GATA-1 target
genes, that the presence of SCL/TAL1 helps distinguish transcriptional activation versus
repression, and that Polycomb Repressive Complex 2 (PRC2) is involved in epigenetic silencing
of a subset of GATA-1 repressed genes. These data provide insights into GATA-1 mediated
gene regulation in vivo. |
| first_indexed | 2024-09-23T08:59:40Z |
| format | Article |
| id | mit-1721.1/60029 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:59:40Z |
| publishDate | 2010 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/600292022-09-30T12:41:18Z Insights into GATA-1 Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis Yu, Ming Riva, Laura Schindler, Yocheved Moran, Tyler B. Cheng, Yong Yu, Duonan Hardison, Ross C. Weiss, Mitchell J. Orkin, Stuart H. Bernstein, Bradley E. Fraenkel, Ernest Cantor, Alan B. Massachusetts Institute of Technology. Department of Biological Engineering Fraenkel, Ernest Riva, Laura Fraenkel, Ernest The transcription factor GATA-1 is required for terminal erythroid maturation and functions as an activator or repressor depending on gene context. Yet its in vivo site selectivity and ability to distinguish between activated versus repressed genes remain incompletely understood. In this study, we performed GATA-1 ChIP-seq in erythroid cells and compared it to GATA-1 induced gene expression changes. Bound and differentially expressed genes contain a greater number of GATA binding motifs, a higher frequency of palindromic GATA sites, and closer occupancy to the transcriptional start site versus non-differentially expressed genes. Moreover, we show that the transcription factor Zbtb7a occupies GATA-1 bound regions of some direct GATA-1 target genes, that the presence of SCL/TAL1 helps distinguish transcriptional activation versus repression, and that Polycomb Repressive Complex 2 (PRC2) is involved in epigenetic silencing of a subset of GATA-1 repressed genes. These data provide insights into GATA-1 mediated gene regulation in vivo. National Institutes of Health (U.S) (P01 HL32262-25) 2010-11-23T16:09:46Z 2010-11-23T16:09:46Z 2009-11 Article http://purl.org/eprint/type/JournalArticle 1097-2765 http://hdl.handle.net/1721.1/60029 Yu, Ming et al. “Insights into GATA-1-Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis.” Molecular Cell 36.4 (2009): 682-695. https://orcid.org/0000-0001-9249-8181 en_US http://dx.doi.org/10.1016/j.molcel.2009.11.002 Molecular Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier E. Fraenkel via Howard Silver |
| spellingShingle | Yu, Ming Riva, Laura Schindler, Yocheved Moran, Tyler B. Cheng, Yong Yu, Duonan Hardison, Ross C. Weiss, Mitchell J. Orkin, Stuart H. Bernstein, Bradley E. Fraenkel, Ernest Cantor, Alan B. Insights into GATA-1 Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis |
| title | Insights into GATA-1 Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis |
| title_full | Insights into GATA-1 Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis |
| title_fullStr | Insights into GATA-1 Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis |
| title_full_unstemmed | Insights into GATA-1 Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis |
| title_short | Insights into GATA-1 Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis |
| title_sort | insights into gata 1 mediated gene activation versus repression via genome wide chromatin occupancy analysis |
| url | http://hdl.handle.net/1721.1/60029 https://orcid.org/0000-0001-9249-8181 |
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