Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy
The tongue consists of a complex, multiscale array of myofibers that comprise the anatomical underpinning of lingual mechanical function. 3-D myoarchitecture was imaged in mouse tongues with diffusion spectrum magnetic resonance imaging DSI at 9.4 T bmax 7000 s /mm, 150- m isotropic voxels ,...
| Main Authors: | , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
SPIE
2010
|
| Online Access: | http://hdl.handle.net/1721.1/60030 https://orcid.org/0000-0003-4698-6488 |
| _version_ | 1811090769876877312 |
|---|---|
| author | Gaige, Terry Kwon, Hyuk-Sang Dai, Guangping Cabral, Victor C. Wang, Ruopeng Nam, Yoon Sung Engelward, Bevin P. Wedeen, Van J. So, Peter T. C. Gilbert, Richard |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Gaige, Terry Kwon, Hyuk-Sang Dai, Guangping Cabral, Victor C. Wang, Ruopeng Nam, Yoon Sung Engelward, Bevin P. Wedeen, Van J. So, Peter T. C. Gilbert, Richard |
| author_sort | Gaige, Terry |
| collection | MIT |
| description | The tongue consists of a complex, multiscale array of myofibers
that comprise the anatomical underpinning of lingual mechanical
function. 3-D myoarchitecture was imaged in mouse tongues with
diffusion spectrum magnetic resonance imaging DSI at 9.4 T bmax
7000 s /mm, 150- m isotropic voxels , a method that derives the
preferential diffusion of water/voxel, and high-throughput 10 fps
two-photon microscope TPM . Net fiber alignment was represented
for each method in terms of the local maxima of an orientational
distribution function ODF derived from the local diffusion DSI and
3-D structural autocorrelation TPM , respectively. Mesoscale myofiber
tracts were generated by alignment of the principal orientation
vectors of the ODFs. These data revealed a consistent relationship
between the properties of the respective ODFs and the virtual superimposition
of the distributed mesoscale myofiber tracts. The identification
of a mesoscale anatomical construct, which specifically links
the microscopic and macroscopic spatial scales, provides a method
for relating the orientation and distribution of cells and subcellular
components with overall tissue morphology, thus contributing to the
development of multiscale methods for mechanical analysis. |
| first_indexed | 2024-09-23T14:51:41Z |
| format | Article |
| id | mit-1721.1/60030 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:51:41Z |
| publishDate | 2010 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/600302022-10-01T22:59:05Z Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy Gaige, Terry Kwon, Hyuk-Sang Dai, Guangping Cabral, Victor C. Wang, Ruopeng Nam, Yoon Sung Engelward, Bevin P. Wedeen, Van J. So, Peter T. C. Gilbert, Richard Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Engelward, Bevin Gaige, Terry Kwon, Hyuk-Sang Dai, Guangping Ruopeng, Wang Nam, Yoon Sung Engelward, Bevin P. Wedeen, Van J. Gilbert, Richard So, Peter T. C. Cabral, Victor C. The tongue consists of a complex, multiscale array of myofibers that comprise the anatomical underpinning of lingual mechanical function. 3-D myoarchitecture was imaged in mouse tongues with diffusion spectrum magnetic resonance imaging DSI at 9.4 T bmax 7000 s /mm, 150- m isotropic voxels , a method that derives the preferential diffusion of water/voxel, and high-throughput 10 fps two-photon microscope TPM . Net fiber alignment was represented for each method in terms of the local maxima of an orientational distribution function ODF derived from the local diffusion DSI and 3-D structural autocorrelation TPM , respectively. Mesoscale myofiber tracts were generated by alignment of the principal orientation vectors of the ODFs. These data revealed a consistent relationship between the properties of the respective ODFs and the virtual superimposition of the distributed mesoscale myofiber tracts. The identification of a mesoscale anatomical construct, which specifically links the microscopic and macroscopic spatial scales, provides a method for relating the orientation and distribution of cells and subcellular components with overall tissue morphology, thus contributing to the development of multiscale methods for mechanical analysis. 2010-11-23T16:42:00Z 2010-11-23T16:42:00Z 2008-11 2008-08 Article http://purl.org/eprint/type/JournalArticle 1083-3668 http://hdl.handle.net/1721.1/60030 Gaige, Terry A. et al. “Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy.” Journal of Biomedical Optics 13.6 (2008): 064005-8. ©2008 SPIE https://orcid.org/0000-0003-4698-6488 en_US http://dx.doi.org/10.1117/1.3046724 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE B. Engelward via Howard Silver |
| spellingShingle | Gaige, Terry Kwon, Hyuk-Sang Dai, Guangping Cabral, Victor C. Wang, Ruopeng Nam, Yoon Sung Engelward, Bevin P. Wedeen, Van J. So, Peter T. C. Gilbert, Richard Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy |
| title | Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy |
| title_full | Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy |
| title_fullStr | Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy |
| title_full_unstemmed | Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy |
| title_short | Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy |
| title_sort | multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy |
| url | http://hdl.handle.net/1721.1/60030 https://orcid.org/0000-0003-4698-6488 |
| work_keys_str_mv | AT gaigeterry multiscalestructuralanalysisofmouselingualmyoarchitectureemployingdiffusionspectrummagneticresonanceimagingandmultiphotonmicroscopy AT kwonhyuksang multiscalestructuralanalysisofmouselingualmyoarchitectureemployingdiffusionspectrummagneticresonanceimagingandmultiphotonmicroscopy AT daiguangping multiscalestructuralanalysisofmouselingualmyoarchitectureemployingdiffusionspectrummagneticresonanceimagingandmultiphotonmicroscopy AT cabralvictorc multiscalestructuralanalysisofmouselingualmyoarchitectureemployingdiffusionspectrummagneticresonanceimagingandmultiphotonmicroscopy AT wangruopeng multiscalestructuralanalysisofmouselingualmyoarchitectureemployingdiffusionspectrummagneticresonanceimagingandmultiphotonmicroscopy AT namyoonsung multiscalestructuralanalysisofmouselingualmyoarchitectureemployingdiffusionspectrummagneticresonanceimagingandmultiphotonmicroscopy AT engelwardbevinp multiscalestructuralanalysisofmouselingualmyoarchitectureemployingdiffusionspectrummagneticresonanceimagingandmultiphotonmicroscopy AT wedeenvanj multiscalestructuralanalysisofmouselingualmyoarchitectureemployingdiffusionspectrummagneticresonanceimagingandmultiphotonmicroscopy AT sopetertc multiscalestructuralanalysisofmouselingualmyoarchitectureemployingdiffusionspectrummagneticresonanceimagingandmultiphotonmicroscopy AT gilbertrichard multiscalestructuralanalysisofmouselingualmyoarchitectureemployingdiffusionspectrummagneticresonanceimagingandmultiphotonmicroscopy |